Study On Preparation Of Erythromycin Nanoemulsion And Its Pharmacy

The objective of this experiment is to prepare oil-in-water erythromycin nanoemulsion and investigate its physicochemical property, stability, security and effect, accordingly offer academic gist for futher investigation of erythromycin nanoemulsion.In the experiment, Tween-80 as surfactants, absolute ethyl alcohol as cosurfactants and acetic ether as oil and deionized water were chosen to sieve the best recipe which was used to prepare erythromycin nanoemulsion by pseudoternary phase diagrams. The particle shape, size and distribution of erythromycin nanoemulsion were detected with transmission electron microscope and Zetasizer Nano ZS. The stability of erythromycin nanoemulsion was investigated by accelerated test in constant temperature and reserved test in different temperature. The content of erythromycin in erythromycin nanoemulsion was measured with ultraviolet spectrophotometry. Its security was evaluated by acute toxicity experiment. Its drug effect was tested by anti - bacterial experiments in vitro and in vivo and clinical test.The results showed that the nanoemulsion area was the largest when the ratio of Tween-80 and absolute ethyl alcohol was 3:1, the ratio of the gross of them and ethyl acetate was 8:2. The nanoemulsion that had been prepared is low viscosity, straw yellow and transparent liquid in appearance, and it presented as small spherical drops under Transmission Electron Microscope (TEM), the diameter of erythromycin nanoemulsion was about 5-20 nm by Zetasizer Nano ZS testing. Erythromycin nanoemulsion was still homogeneous and transparent. Moreover, it didn't stratify and subside after centrifuging for 20min with 10 000rpm and reserving under -4℃, room temperature and 60℃for 6 months.Linear relation was all right and r was 0.9932 when the concentration ranged from 75 to 175 ug·mL-1, the average recovery rate was 98.85%, and RSD was 0.19%, the inter-day and intra-day precision of determination of erythromycin was less than 1%. And the content of erythromycin in nanoemulsion was 8.557 mg /ml. Acute toxicity experiment showed that the toxicity of erythromycin microemulsion belonged to practically low-toxic substance; Anti-bacterial experiments in vitro showed that the MICs of erythromycin nanoemulsion on staphylococcus and streptococcus were respectively 0.01563 and 0.125 mg/mL,the MICs of erythromycin Enteric-coated tablets on staphylococcus and streptococcus were respectively 0.03125 and 0.25 mg/mL,the MICs of roxithromycin tablets on staphylococcus and streptococcus were respectively 0.01563 and 0.25 mg/mL,which showed that the antibacterial effect of erythromycin nanoemulsion on staphylococcus and streptococcus in vitro was remarkable; Anti - bacterial experiments in vivo showed that the efficacy of erythromycin nanoemulsion against experimentally induced staphy-locosis in chicken was better than erythromycin enteric-coated tablets;Clinical test showed that the the clinical effect of erythromycin nanoemulsion on avian chronic respiratory disease was good.From the results above, conclusion can be achieved that the nanoemulsion prepared correspond to the standard of nanoemulsion and has fine stability, security, effect.