The Relationship Of Helicobacter Pylori Infection And The Expression Of Interleukin-10 And Alpha-Catenin In Gastric Cancer

OBJECTIVE:To investigate the expression and the clinical significance of Alpha-Catenin(α-Cat)and interleukin-10(IL-10)in human carcinogenesis of gastric mucosa and its relationship with Helicobacter pylori(H pylori) infection.METHODS:SP immunohistochemical technique was used to detect the expression ofα-Cat and IL-10 in pathological samples,including 96 cases of gastric carcinoma,96 cases of gastric precancerous and 15 control group.Rapid urease test and histological examination with Warthin-Starry were used to determine the status of H pylori infection.RESULTS:The positive rates ofα-Cat expression in the sample of gastric cancer,adjacent tissues and control group were 34.4%,56.2%,100% respectively.(P＜0.05);The positive rates of IL-10 expression in the sample of gastric carcinoma,adjacent tissues and control group were 63.5%,48.9%,13.3% respectively(P＜0.05);There was significant difference between the positive expression of H pylori infection and negative expression of H pylori infection in GC(71.8%vs.28.2%,P＜0.05).The expression ofα-Cat in GC with H pylori positive was markedly lower than that in H pylori negative ones(36.2%vs. 51.8%,P＜0.05).The expression of IL-10 in H pylori positive GC tissues was notably higher than in H pylori negative ones(47.8%vs.29.6%,P＜0.05).The positive rate ofα-Cat in gastric cancer tissue was positively paralleled with the increase of differentiation grade,but no significant differences existed between depths of invasion and lymph node metastasis(P＞0.05);the positive rate of IL-10 in gastric cancer tissue was negatively paralleled with the increase of differentiation grade,but no significant differences existed between different depths of invasion and lymph node metastasis(P＞0.05).CONCLUSIONS:The expression ofα-Cat was gradually down-regulated and conversely the expression of IL-10 was progressively up-regulated during the course of gastric carcinogenesis.H pylori infection is closely related to the expression ofα-Cat and IL-10 in GC.H pylori infection may be the initiating factor,and the activation ofα-Cat and IL-10 may be one of the key events in the pathogenesis of GC.H pylori infection,IL-10 activation,andα-Cat expression are playing synergic roles in the occurrence of GC.