The Research Of Animal Experiments For Bronchoscopic Lung Volume Reduction Surgery With The One-way Valve

Objective Explore the therapy effects of emphysema dogs through bronchoscope put into the one-way valve .Methods Eight Beagle dogs half male and half female, 2 years old, weighing 13-18 kg, aerosol papain 12,000 U / kg, 1 / weeks, a total of two weeks, reproduction emphysema model to CT and pulmonary function test and execution experimental animals only from specimens that have become pathological obstructive pulmonary emphysema model, in the remaining seven dogs average placement in animals right lung three valves that designed by our own, two months two months later chick animals chest CT and pulmonary function and then executed experimental animals, specimen observation, evaluation and light microscopic pathology computer image analysis, and objectively evaluate efficacy. Results: The animals had no significant adverse reactions, apart from the two valve coughed up, the remaining 19 have not shift location. CT revealed the corresponding valve implanted lung lobe occurred pneumonia, pulmonary fibrosis performance. In functional residual capacity (FRC), lung volume (TLC), functional residual capacity ratio total lung capacity (FRC/TLC%), tidal volume (VT) and respiratory frequency (BR), the model of emphysema ago (before making model), after the results were between statistical differences significance (P <0.05); after the model of emphysema (after making model) and Two months after surgery VT, FRC, TLC difference was statistically significant (P <0.01), FRC / TLC% BR and no difference between statistical significance (P> 0.01); before making model and Two months after surgery FRC / BR TLC% which the difference was statistically significant (P <0.05), VT, FRC, TLC no difference between statistical significance (P> 0.05). Apart from two valve cough off, the remaining 19 have not shift location. Experimental animals have no obvious adverse reactions. Specimens observed that the one-way valve obstructed lobe became narrow,hard texture, visceral pleura a focal scar formation, bronchial wall around a little hyperplasia; The organizations around lobe have no hemorrhage and solidified. Pathological observation and image analysis results showed that seven dogs have been blocked lobe moderate alveolitis and lung fibrosis performance. Quantitative image analysis showed that, compared with emphysema model dogs, seven dogs have significantly closed alveolar depression, reduced lung volume performance (P <0.01), compared with normal dogs alveolar subsidence also closed, reducing performance of lung volume (P < 0.05).