| Objective:To investigate the expression of Pim-3, phosphorylated STAT3 (p-STAT3) and CyclinD1 protein by high-throughput tissue microarray technique, and their relationship with the clinicopathologic parameters in pancreatic ductal adenocacinoma (PDAC).Methods: The pancreatic carcinoma tissue microarray was composed of 88 cases of the pancreatic adenocarcinoma and 11 cases of noncancerous tissues as control subject. Immunohistochemistry was performed to explode the expressions of Pim-3, p-STAT3 and CyclinD1. The relationship between their expression and the clinicopathologic parameters, and the survival time of 54 cases, which were follow-up from 2 months to 6 years were analyzed.Results: The positive rates of Pim-3, p-STAT3 and CyclinD1 in 88 cases of pancreatic carcinoma were 68.4%,70.5% and 70.5% respectively, the expression of Pim-3 and p-STAT3 were negative in noncancerous group, while CyclinD1 was 18.2% in noncancerous group. Pim-3 in pancreatic carcinoma was located in the cytoplasm, while Cyclin D1 was located in the cell nucleus, and p-STAT3 was located in the cytoplasm and cell nucleus. Positive expresssion of Pim-3, p-STAT3 and CyclinD1 were closely correlated to the TNM clinical stage and lymph node metastasis(P<0.01), but had no correlation to patients'age, sex, tumor size, location and grade(P>0.05). Spearmen rank correlation showed a significant positive correlation was observed among this three indexes(P<0.05). The overexpression of Pim-3,p-STAT3 and CyclinD1 were significantly related to short survival(P=0.001, P=0.000, P=0.016). Cox multivariate analysis revealed that Pim-3 and p-STAT3 were prognosticators for PDAC(P=0.043, P=0.046). Conclusion:Tissue microarray technique is an effective method to detect multiple gene protein expression. The overexpressions of Pim-3, p-STAT3 and CyclinD1 closely correlated with the clinical stage and metastasis of pancreatic carcinoma. Pim-3 and p-STAT3 may indicate poor prognosis of PDAC.
|
PDF Full Text Request