Expression Of Inhibitor Of Apoptosis Protein Livin In Transitional Cell Carcinoma Of Bladder

Livin is a new member of Inhibitor of Apoptosis Protein (IAP), livin was highly expressed in the most of the malignant tumors, but seldom found in normal tissues. Livin is a kind of nucleolus proteins which can inhibit the apoptosis of cells. Livin can indirectly inhibit the activity of caspase-3, 7, 9 and block the transduction of dead signal by bonding with it. Because of this, this work can conclude that livin is the key point in the progress of inhibiting of medicine induced apoptosis. This work team has been using RT-PCR method to detect the expression of livin in Transitional Cell Carcinoma of Bladder (TCC), initially proved livin played an important role in tumor occurrence and progress. This study will detect the expression of livin in the level of protein from the TCC.Objective: This study is aimed at researching the expression of livin,a novel Inhibitor of Apoptosis Protein(IAP)family member in Transitional Cell Carcinoma of Bladder (TCC) and exploring the relationship between the expression level of livin and the grading and staging of TCC.Methods: 1,Frozen bladder tissue array-based immunohistochemical Staining (IHC) was used to examine the pattern of livin protein expression in 50 tissue spots including 40 cases of bladder cancers and 10 noncancerous mucosa. 2,the way of qualitative and semiquantitative of Western blotting was adopted to examine the expression of livin in overall 40 samples from the group of TCC and 10 samples from the group of noncancerous mucosa.Results: 1,Immunohistochemistry:Livin was expressed in 34 of 40 TCC case(85%). No livin expression was detected in all 10 normal bladder mucosa. There is statistically significant between them (P<0.01). There is statistically significant between the group of invasion and the group of superficial, but there is not statistically significant between the three pathologic grades. 2,Western blotting was used to detect the expression of livin in 40 cases(34 men and 6 women) of TCC and 10 cases of normal bladder tissues(control). There are 37 utility samples of the whole 40 TCC cases, and livin was expressed in 35 of 37 TCC cases (95%). There was negative in the control group. The positive rate of livin between TCC and control was significant statistically (P<0.01).After using gray scale analysis of livin and F test we proved that there is statistically significant between the four groups of TCC cases (F=5.926, P=0.003).The result proved that there is statistically significant between the clinical stages and recurrence. Livin was no correlated with the grade and quantity,(P>0.05).Conclusion: The higher expression of livin in TCC suggests that livin plays an important role in tumor occurrence and progress through inhibiting cell apoptosis.Our results indicated that livin was no correlated with the grade and quantity., but correlated with the stage and recurrence. Therefore, we conclude that livin may be the index for identifying early stage of TCC. Livin may provide a new appropriate target for gene therapy to TCC.