| Objective: To evaluate the biocompatibility of porous silk fibroin/ hydroxyapatitecomposite artificial bone and to investigate the feasibility of SF/HA as a scaffold for the bone tissue engineering.Methods: Bone marrow stromal cells (BMSCs) are collected from rabbits, cultured ,identified, induced, and to be identify osteogenesis characteristics; The induced BMSCs of rabbit are cultured on SF/HA and the biological characteristics of the cells are examined in vitro and in vivo to evaluate the biocompatibility of porous silk fibroin/hydroxyapatite composite artificial bone.Result: Bone marrow stromal cells (BMSCs) which are collected from rabbits, cultured, induced and proliferated in vitro. The expressions of calcium nodes are seen by Von Kossa staining. The induced BMSCs are co-cultured with SF/HA. We observe the cells could beattached to, extended to and show their proliferated abilities normally on SF/HA using inverted microscope, scanning elec-tronmicroscope and MTT test. The alkaline phosphatase activity shows no manifest difference after alkaline phosphatase assay by means of modified calcium-cobah staining. By using flow cytometry (FCM), the use of two different materials have no effect on cell cycle cells. The induced BMSCs are cultured in extraction solution of SF/HA to evaluate the cytotoxicity and cytotoxicity stage of porous materials was 0 or 1. The intramuscular implantation induce no muscular degeneration, necrosis or obvious inflammatory reaction. The tissue of liver and kidney have no degeneration and necrosis at the different time points.Conclusion: The results show that porous silk fibroin/hydroxyapatite composite artificial bone is well biocompatible with BMSCs, and it would be a good scaffold for the bone tissue engineering. |
PDF Full Text Request