Morphological Observation Of Hippocampal CA1 Area In Mice With Forebrain Ischemia Reperfusion

Objective: To establish a experimental model for evaluating the effect of remedy for denatured neurons after cerebral ischemia-reperfusion.Methods : Sixty mice were randomly divided into six groups: sham operation group (A), ischemia for 30 min (B), 60 min (C), 90 min (D), 120 min (E) groups, mannitol treated group (F). Mannitol treated group was immediately injected mannitol after 120 min ischemia. To decide suitable ischemia time and whether the model would be feasible or not, morphological changes of hippocampal CA1 area were compared between 6 groups after 24 h reperfusion.Results: Reperfusion time invariable, the cell was injured more and more seriously as ischemia time prolongs from zero to 120 min. Cerebral ischemia-reperfusion injury can be obstructed or healed by injecting mannitol. Compared with group A, the number of living cell in groups C, D, E were obviously less (P average< 0.01) . Group F was higher than group E (P average< 0.01) , but there was no difference between group F and A (P average > 0.05) . Compared with group A, the denatured neurons rates of hippocampal CA1 area in groups B, C, D and E were higher (P average < 0.05). Similarly, Group F is less than group E(P average < 0.05). There was no significant difference between group A and F (P average > 0.05). The proportion of diverse injured neurons in group C was in balance.Conclusions: 1. The mouse model of 60 min ischemia and 24 h reperfusion is low cost, convenient in making and nice in reproducibility and can provid a region in where denatured neurons exist intensively. 2. The model can be used to evaluate the remedies for denatured neurons after ischemia reperfusion. 3. HE stain just can do simple evaluation on cell morphous. If possible, by employing Confocal microscopy or immunohistochemistry, we would evaluate the function of cells. 4. It is the next objective to observe morphological changes after more than 24 h reperfusion.