Intervention Of Xuefu Zhuyu Oral Liquid On Expression Of Adhesion Molecule CD11b/CD18,CD54 In Neutrophils In Patients With Ateriosclerosis Obliterans

PartⅠobserve the expression of adhesion molecular CD11b/CD18,CD54 in peripheral neu-trophils and its relation with arteriosclerotic obliterans(ASO)Objective To observe the expression of adhesion molecular CD11b/CD18,CD54 in peripheral neutrophils and its relation with arteriosclerotic obliterans(ASO).Methods Flow cytometery analysis was used to detect the expression of CD11b/CD18,CD54 in peripheral neutrophils of 30 patients with ASO and 30 healthy subjects by direct immunofluroscent technique.Results The expression of CD11b/CD18,CD54 in neutrophils in ASO patients was significantly higher than that in the healthy subjects(P＜0.05) and stepped in keeping with the severity of the disease.PartⅡstudy the effect of Xuefu Zhuyu Oral Liq-uid(XZOL) on the expression of adhesion molecular CD11b/CD18,CD54 in peripheral neutrophils and its relation with arteriosclerotic obliterans(ASO).Objective To study the effect of Xuefu Zhuyu Oral Liq-uid(XZOL) on the expression of adhesion molecular CD11b/CD18,CD54 in peripheral neutrophils and its relation with arteriosclerotic obliterans(ASO).Methods Neutrophils were separated from whole blood of ASO patients and cultured, CD11b/CD18 were detected after the cultured cells were interfered with XZOL dilution at different time points(1h,6h,12h).Results The expression of CD11b/CD18 in neutrophils in ASO patients was significantly lowered in the treatedgroup 6 and 12 h after XZOL intervention,showing significant difference as compared with that in the control group and the level 1 h after medication(P＜0.05).Conclusions CD11b/CD18,cCD54 may involve in the pathogenesis of ASO and be related to the severity of arteriosclerosis.The possible mechanism of XZOL in treating and preventing ASO might be through reducing the expression of CD11b/CD18,CD54 in peripheral neutrophils to interfere the adhesive function of them.