The Potential Of Dendritic Cells-derived Exosomes In Immunotherapy Of Chronic Hepatitis B Infection

AIMTo determine whether dendritic cell-derived exomes loaded with Hepatitis B core antigen could induce the proliferation of autologous lymphocyte .METHEDSDCs were generated from PBMCs( peripheral blood monouclear cells )of healthy donors. Exosome secreted from DCs were purifed by ultracentrifugation and the molecules of exosome were identited by western blotting. Autologous lymphocyte proliferation assays and ELISPOT were used to evaluate the efficacy of DEXs loaded with HbcAg.RESULTMature DCs had a higher expression of HLA-DR,CD83 and costimulatory molecules CD80/CD86 compared to immature DCs(45.3±8.3% vs 16.5±6.8%,83.53±10.8% vs 4.3±2.8%,90.2±6.5% / 92.38±13.6% vs 6.3±4.5%/49.8±9.2%,P<0.05). Accordingly, Mature dendritic cell-derived exosome had a higher expression of HLA-DR and CD86 compared to imDEXs. Mature DEXs loaded with HbcAg showed a greater capacity for autogolous lymphocyte proliferation than imDEXs(OD 0.904±0.003 vs 0.545±0.010,P<0.001). Mature DEXs can also induce IFN-γproduction in PBMCs without addition of DCs(sfc/2×105PBMC 32±13 vs imDEXs 14±2,P<0.05).CONCLUSIONSDEXs pulsed with HbcAg can initiate and amplify the antigen-specific T lymphocytes-base immune response, DEXs as potent cell-free peptide-based vaccine, will be expected to be used for immunotherapy of chronic HBV infection.