| Objective: To study the feasibility of applying immunohistochemical assay in coronary collateral development in mini pigs, which will be methodological foundation in mechanism of cardiac rehabilitation.Methods: An animal model of controllable myocardial ischemia was established in 45 mini pigs. The animals were equally divided into the sham-operated group (SO), sedentary ischemic group (SI) and exercise training group (ET). Repeated transient myocardial ischemia was induced by inflation of balloon constrictor or exercise training for 8 weeks. The myocardium attributed to the oblique marginal artery was finally sampled at the end point of the study for measurement of capillary density (CD). The methods of the measurement were H.E. staining and immunohistochemical assay labeled by Factor VIII -RA. The reproductivity of the two methods for CD analysis was studied.Results: Both methods demonstrated significant increase of myocardial CD in ET, which was significantly higher than SI (P<0.01) and SO (P<0.01). Furthermore, the myocardial CD in SI was also higher than SO (P<0.01). The CD analyzed by immunohistochemistry was higher than H.E. (P<0.05). The reproducibility between H.E. staining and immunohistochemical assay was found excellent (P>0.05). Coronary collateral blood flow (CCBF) was positively correlated with CD analyzedby both methods. The correlation coefficient betweenimmunohistochemical assay and CCBF was significantly higher than H.E.staining (P<0.05).Conclusion: Immunohistochemical assay is an accurate and reliablemethod for quantitative analysis of the coronary collateral developmentin mini pig. |
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