Influence Of Adiponectin On The Expression Of CD55 And CD59 On HUVECs

Object To observe influence of adiponectin on the expression of CD55 and CD59 on human umbilical vein endothelial cells(HUVECs).Methods Cultivate HUVECs in vitro. HUVECs were divided into three groups—CRP, adiponectin and blank control. HUVECs were respectively incubated with 50ug/ml CRP and 25ug/ml adiponectin for 48 hours.CD55 and CD59 expression on HUVECs were detected by flowcytometry.Results After CRP stimulated HUVECs, CD55 mean fluorescence intensity,CD59 mean fluorescence intensity and CD55 percentage were increased compared with blank control. (3.84±0.21 versus 2.81±0.06, p<0.001; 69.72±4.48 versus 51.15±7.86, p<0.01; 98.30±1.13 versus 93.57±1.18, p<0.001) .After adiponectin stimulated HUVECs,CD59 mean fluorescence intensity and CD55 percentage were increased compared with blank control ( 63.02±4.90 versus 51.15±7.86, P<0.05: 96.55±0.66 versus 93.57±1.18, P<0.01) .Conclusion Adiponectin can upregulates expression of complement regulatory protein CD55 and CD59, and which may contributes to the anti-inflammation and anti-atherosclerosis effect of adiponectin.