The Study And Application Of CE In The Analysis Of Traditional Chinese Medicines And Their Decoctions

Natural medicinal resources provide valuable material for the discovery and development of new drugs of natural origin. The large numbers of other components in traditional Chinese medicines (TCMs) make the screening and analysis of the bioactive components extremely difficult. In the past decades, many effective analysis tools have been used for analyzing the components of TCMs in order to control the quality and discover bioactive compounds. Capillary electrophoresis (CE) is a new analysis technology. It provides advantages in terms of higher efficiency, faster separation time, less injected sample volume and more practicable operating modes. The advantages of the combined flow injection with capillary electrophoresis (FI-CE) system have been demonstrated not only to produce an efficient and reliable mode of sample introduction with improved throughput and precision and outstanding reproducibility of retention time, peak area and peak height, but also to provide on-line sample pretreatment ability. In the second part of this dissertation, the advantages of CE and FI-CE were made use of to separate and determinate active components in the decoction of Glscyrrhiza uralensis Fisch.On the basis of the previous literatures, in this dissertation, some original studies are carried out as followings:1. A novel method based on capillary zone electrophoresis (CZE) was developed for determining glycyrrhetinic acid (GTA) and glycyrrhizic acid (GA) in Glycyrrhiza uralensis Fisch root. This method has also been successfully applied to determine the optimum time of the steeping process and decocting process of Glycyrrhiza uralensis Fisch root.2. The flow injection-capillary electrophoresis (FI-CE) system was used in connection with an on-line decoction system to automatically monitor glycyrrhetinic acid (GTA) and glycyrrhizic acid (GA) in the decoction process of Glycyrrhiza uralensis Fisch root for the first time.3. A novel microemulsion electrokinetic chromatography (MEEKC) method was established for simultaneous determination of Imporation and Osthol in the medicinal plant Fructus Cnidii.This dissertation consists of three parts and five chapters.Part I Review of the development of capillary electrophoresis and application of CE in Traditional Chinese Medicines and their decoctions.Chapter 1: The principle and characteristics of CE were simply described. The application of CE in study of TCMs and the application of analytical methods in the decoction of TCMs were reviewed and discussed in details.Part II Study and application of CE in the bioactive components of Traditional Chinese Medicines.Chapter 2: A fast, sensitive and precise analytical method based on CZE was developed for determining glycyrrhetinic acid (GTA) and glycyrrhizic acid (GA) in Glycyrrhiza uralensis Fisch root. This analysis was carried out with 10 mM borate (adjusted to pH 8.8) as a background electrolyte. The separation was achieved in 5 min. This method has also been successfully applied to determine the optimum time of the steeping process and decocting process of Glycyrrhiza uralensis Fisch root.Chapter 3: The FI-CE system was used in connection with an on-line decoction system to automatically monitor glycyrrhetinic acid (GTA) and glycyrrhizic acid (GA) in the decoction process of Glycyrrhiza uralensis Fisch root. The separation of GTA and GA took less than 5 min by using a 10 mM borate buffer (adjusted pH to 8.8). The dynamic changes of GTA and GA on the decoction time were obtained in the on-line decoction process of Glycyrrhiza uralensis Fisch root.Chapter 4: A rapid and effective MEEKC method was established for simultaneous determination of Imporation and Osthol in the medicinal plant Fructus Cnidii. After optimization of separation conditions, baseline separation was obtained for the two analytes within 20 min using a running buffer composed of 20 mMTris +20% (v/v) microemulsion stock solution(16.55%(w/v) SDS + 40.75%(v/v)l-butanol + 5.85%(v/v) n-heptane + 36.85%(v/v) water). The proposed method is a good alternative for simultaneous analysis of coumarins compounds in other medicinal plants.Part III Study and application of CE in enantioseparation of four stereoisomersof palonosetron.Chapter 5: The enantioseparation of four stereoisomers of palonosetron hydrochloride by CZE usingβ-CD as chiral selector was described for the first time.β-CD was shown to be effective in separating palonosetron hydrochloride stereoisomers. Baseline separation of palonosetron hydrochloride stereoisomers was achieved within 35 min using a running buffer composed of 30 mM NaH₂PO₄+10% (v/v) methol+150 mMβ-CD(adjusted pH to 3.0)...