Expression Of Cdt1 And Geminin In Leukemic Cell And Distribution Of Every Phase In Cell Cycle

Section I The expression of Cdt1 and Geminin in Jurkat cellObjective To explore the expression of DNA replication licensing factor Cdt1 and the DNA replication inhibitor Geminin in leukemic cell in order to provid evidence for pathogenesy, diagnose, treatment and prognosis of leukemia.Methods Cultivate leukemic cell of Jurkat in vitro,exponential phase of growth Jurkat cell(research group) and peripheral blood leukomonocyte from health children(control group). We detected the expression of Cdt1 and Geminin mRNA by RT-polymerase chain reaction(RT-PCR). The outputs are semiquantitative analysised. The Cdt1 and Geminin proteins are detect by flow cytometry . The expression of proteins are quantitative analysised by mean fluorescence intensity.Resultsâ‘ Cdt1 mRNA and Geminin mRNA are all highlier expressed in research group than control group (P<0.05) .â‘¡Cdt1 proteins and Geminin proteins are all highlier expressed in research group than control group (P< 0.05)Conclusionsâ‘ The over-expression of Cdt1 in in Jurkat cells(human T-lymphocytic leukemic cell lines) may be releated to the maligmant proliferation of leukemic cells.â‘¡The over-expression of Geminin in Jurkat cells(human T-lymphocytic leukemic cell lines) may be involved in the maligmant proliferation of leukemic cells.Section II The expression of Cdt1 and Geminin in every phaseof cell cycle in K562 cellObjective To explore the expression of DNA replication licensing factor Cdt1 and the DNA replication inhibitor Geminin in every phase of cell cycle of leukemic cell.Methods Cultivate leukemic cell K562 in vitro. K562 cell is synchronized by thymidine.The efficiency of synchronization is detected by flow-cytonetry(dying by PI).The Cdt1 and Geminin proteins are detected by flow cytometry . The expression of proteins are quantitative analysised by mean fluorescence intensity.Resultsâ‘ K562cells are arrested at G1-to-S transition phase by thymidine for 24 hours.Cells entry in S phase at 4 hour after release.Most cells entry in G2/M phase at 12 hour after release.Cells recover to the state of exponential phase of growth K562 cells.â‘¡Cdt1 and Geminin proteins are expressed during all the phases of cell cycle in K562 cell.â‘¢There are significant deviations in the levels of Cdt1 and Geminin proteins in every phase of cell phase (P<0.05) .Conclusionsâ‘ Cdt1 and Geminin proteins are expressed during all the phases of cell cycle in K562 cell.â‘¡The distribution of Cdt1 protein is abnormal in the every phase of cell cycle in K562 cell may lead to abnormal proliferation of leukemic cells.â‘¢The distribution of Geminin protein is approximately normal in the every phase of cell cycle in K562 cell.