| OBJECTIVE: To explore the effects of adiponectin on diabetic macroangiopathy, we observed the changes of serum lipids, serum soluble intercellular adhesion molecular (sICAM-1) levels and nuclear factor-κBp65 (NF-κBp65) expressions in aorta after transferring rAAV2/1-Acrp30 to atherosclerotic GK rats.METHODS : We set up type 2 diabetic atherosclerotic rat models by feeding GK rats with high-fat diet and L-N-arginine-methylesten intragastric administration. A total number of 30 atherosclerotic GK rat models were randomly divided into three groups:①Model group one (M1): hind limb intramuscular injection of normal saline;②Model group two(M2): hind limb intramuscular injection of vacuity virus rAAV2/1 ;③Treatment group(T): hind limb intramuscular injection of rAAV2/1-Acrp30 with a dose of 1×1012 v.g/ml. After 8 weeks, we measured serum lipids, sICAM-1 levels and aortic NF-κBp65mRNA expressions in each group. Aortic NF-κBp65mRNA expression was measured by RT-PCR.RESULTS:1. After 8 weeks of high-fat diet plus L-NAME intragastric administration, characteristics changes of atherosclerosis were observed in aortas of model rats, such as massive foam cells, swell and discontinuous endothelial cells. 2. Compared with control model groups (M1 and M2), levels of TG,TC,LDL-C in treatment (T) group were decreased significantly (p <0.05) , while levels of HDL-C increased significantly in group T (p <0.05).3. Compared with control model groups (M1 and M2) , sICAM-1 levels in treatment (T) group were decreased significantly (P<0.05) .4.Compared with control model groups (M1 and M2) , aortic NF-κBp65 mRNA expressions were significantly down-regulated in the treatment (T) group (P<0.05) .CONCLUSIONS:1. Rat models with diabetic atherosclerosis can be established by feeding GK rats with high-fat diet and L-N-arginine-methylesten intragastric administration.2. rAAV2/1-Acrp30 may produce protective effects on diabetic atherosclerosis by modulating lipids metabolism and decreasing inflammatory reactions.
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