| Objective: To explain the reasons of T cell with weak immuno- competence in HCC , we analyzed the expression of the correlative factors about activated T cell by immunohistochemistry. Mehtod: The costimulatory molecule B7-1 gene and MHC class I chain-related gene A were both key gene in activated T cell , then 108 cases of HCC were blotted by immunohistochemistry of SP method, analyzed the expression semiquantitative in hepatocytes near the tumour, hepatomas, and the cells in stroma, studied the difference in groups of histologic stage, clinic stage, child-pugh classification and the survival years without tumor; the correlative analysis of hepatitis in serum by ELISA . These cases were the hepatectomy since 2002 from 2005. Then 20 cases were selected randomly, blotted by immunohistochemistry (CD3,CD4,CD8,CD56,CD68,CD20). According to the correlative analysis of hepatitis, we picked out the relative virus and grouping. At last they were analyzed by statistics software. Result: B7-1 and MICA gene were both expressed in hepatocytes, hepatoma cells and part of cells in stroma. They were correlative between hepatocytes and hepatoma cells (rs=0.31, P=0.001; 0.55, P=0.000) . The expression of B7-1 gene was not different in hepatocytes, hepatoma cells,but in clinic stage group the positive rate in group I was higher than group II (x~2=6.58,P<0.05; x~2=8.09 , P<0.005) in hepatoma and in hepatocytes near the tumour. The expression of B7-1 gene was increased following the extension of neoplastic survival in both hepatoma cells and cells in interstitial substance (X~2=4.81, 4.29, P<0.05). The positive rate was raised from 57.69% to 81.82% and from 26.92% to 52.27%. In classification of histology was decreased (x~2= 71.05, P <0.005 ) . The expression of MICA gene in hepatocyte was more obviously than in hepatoma cells (X~2=4.74, P<0.05). The rate of MICA in hepatocytes and hepatoma cells was decreased by the growth of clinic stage (X~2 = 9.44, P<0.01; X~2 = 6.93, P<0.05). But it has no statistic significance in neoplastic survival group . The rate of MICA gene of hepatocytes in group A (58.70%) was less than group B (86.36%) (X~2=4.02, P<0.05 = . The positive rate was different in histological classification (X~2=15.85, P<0.005 = . The cells with single nuclear in interstitial substance were almost positive for CD3, partly positive for CD8,CD20, a few of them positive for CD56,CD68, few of them positive for CD4. The correlation between B7-1 gene, MICA gene and CD3, CD8, CD20 was positive. The cells in stroma of hepatoma were less than in hepatocyte by statistics (P<0.001).The rate of B7-1 gene in group A (56.52%) was higher than group B (27.27%) (X~2=5.11, P<0.025 for telling the the effect of HBeAb by comparison; but was no difference between in hepatocytes and in hepatoma cells (X~2=3.77, 0.01, P>0.05). The effect of HBeAb by comparison, the positive rate in group A (58.70%) was higher than group B(86.36%) (X~2=4.02, P<0.05 . But it has no difference in hepatoma cells and lymphocytes in interstitial substance (X~2=0.09, 0.23, P>0.05).The expression of MICA in stroma was negative correlation with HBsAg (rs=0.39, P=0.006). Being the HBV persistent infection, the expression of B7-1 gene was higher in hepatoma cells (X~2=5.366, P<0.025 ) , but it has no difference in there groups of MICA. Only the B7-1 gene was an independent influence factor for the prognosis. Conclusion: B7-1 and MICA gene were both correlative factors with the prognosis, but only the B7-1 gene was an influence factor independently for the prognosis. B7-1 gene was the important factor of T cell activated in HCC. But without CD4+T cells the immunoreaction was weak reaction only. So adding CD4 positive cell rulely might increase the antineoplastic action. Our study showed that B7-1 gene and HBsAg+HBcAb, HBeAb have synergic action for antineoplastic action. MICA in hepatocytes has antagonism against HBeAb, MICA in stroma was negative correlation with HBsAg. |
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