Study On The Relationship Between Expression Of COX-2, Survivin And Microvessel Density In Meningioma

Objective: To investigate the expression of Survivin, COX-2 in meningioma and their correlation with tumor mi-crovessel density (MVD); By studying their mechanism of molecular biology during the genesis of tumor, we could supply valuable basis of early diagnosis and the prognostic indicator for the clinic and new target gene for the gene therapy of brain astrocytoma.Methods:1 Streptavidin-peroxidasebiotin immunohistochemistry t-echnique was used to detect the expression of COX-2, Sur-vivin and MVD (labled by CD34) in 53 cases of meningi-omas and the relationship between the expression of Survi-vin, COX-2, MVD and the clinicopathological features in meningiomas, the correlation among the expression of COX-2, Survivin and MVD were analyzed statistically.2 The determination of Survivin and COX-2: If the c-ell specific spot appeared the yellowish brown kerneland, higher dyeing intensity than the background nonspecificity dyeing, the cell was positive. The judgment standard refer-ed to the semi-quantitative method which was reported by literature; The determination of MVD used the blood vess-el endothelial cell marked by CD34, which was reported by Weidner.3 All data were analysized by SPSS 11.5 for Windo-ws.Results:1 The results of meningioma pathology graduation: A-ccording to WHO's newest classifycation standard of centr-al nervous system tumor in 2000, We made the pathologic-al diagnosis of the meningioma specimen which had been carried on HE coloration: there are 30 cases of GradeⅠ, 15 cases of GradeⅡ, 8 cases of GradeⅢ.2 The expression of Survivin in meningioma and it's relationship with clinical parameters: In 53 cases of meni-ngiomas, the positive rate of Survivin protein was 56.6% (30/53), and the positive rates were 40%, 73.3%, 87.5% ingradeⅠ,Ⅱ,Ⅲrespectively. The positive rates had progr-essive increasing tendency, which showed that the positive expression of Survivin protein and the histopathology grad-uation of meningioma were closely assosiated using the S-pearman rank correlation analysis (r=0.494, P<0.01). The chisquare test showed that there was a markable differencebetween the positive expression of Survivin protein and themalignant degree of meningioma (P<0.05): The positive ra-te in gradeⅡwas significantly higher than that in gradeⅠ(x2=8.873, P<0.05), higher in gradeⅢthan in gradeⅠ (x2=11.793, P<0.01), but there was no significant difference between gradeⅢand gradeⅡ(x2=2.365, P>0.05) and no distinct difference was found in age, sex and tumor siz-e (P>0.05).3 The expression of COX-2 in meningioma and it's r-elationship with clinical parameters: In 53 cases of mening-iomas, the positive rate of COX-2 protein is 52.8% (28/53), and the positive rates were 43.3%, 60%, 75% in gradeⅠ,Ⅱ,Ⅲrespectively., The positive rates had progressive in-creasing tendency, which showed that the positive expressi-on of COX-2 protein and the histopathology graduation of meningioma were closely assosiated using the Spearman ra-nk correlation analysis (r=0.404, P<0.01). The chisquare te-st showed that there was a markable difference between th-e positive expression of COX-2 protein and the malignant degree of meningioma (P<0.05): The positive rate in gradeⅡwas significantly higher than that in gradeⅠ(x2=10.193,P<0.05), higher in gradeⅢthan in gradeⅠ(x2=17.005, P<0.01), but there was no significant difference between gradeⅢand gradeⅡ(x2=4.527, P>0.05) and no distinct diff-erence was found in age, sex and tumor size(P>0.05).4 The expression of MVD in different ranks of meni-ngioma: The single factor variance analysis carried on stat-istics analysis between the MVD value of each meningiom-a grade showed that the expression of MVD value and themalignant degree of meningioma had the remarkable differ- ence.5 The relationship between the expression of Survivin and COX-2 in meningioma: The positive expression of Su-rvivin and COX-2 was associated with each other (r=0.429,P<0.01).6 The relationship among the expression of Survivin, COX-2 and MVD: Grouped by positive or negative expres-sion of Survivin, the value of MVD in positive group wassignificantly higher than that in negative group (P<0.01); Grouped by positive or negative expression of COX-2, the value of MVD in positive group was significantly higher than that in negative group (P<0.01); the MVD value is t-he highest in the group which Survivin and COX-2 expres-sed positively at the same time (34.8111±11.02414); The S-urvivin negative, the COX-2 positive group or the Survivinpositive, the COX-2 negative group were the next (21.8500±5.87391, 22.9583±8.50791); the MVD value was the low-est in the group which Survivin, COX-2 expressed negativ-ely at the same time (12.1154±3.51872); obviously, the M-VD value in the group which Survivin and COX-2 expres-sed positively at the same time (34.8111±11.02414) was hi-gher than the group which expressed negatively simultaneo-usly (12.1154±3.51872), the statistics had significant differe-nce (P<0.0l).Conclusions:1 The expression of Survivin, COX-2 in meningioma was highly and closely associated with pathological grade.2 Positive correlation between the expression of Survi-vin and COX-2 was observed, which pointed out that theremay be some uncertain mechanism of coordination or inte-rface regulation existing in the development of meningiom-a.3 There was positive correlation of the expression bet-ween Survivin and MVD, or between COX-2 and MVD inmeningioma. The MVD value in both Survivin(+) and CO-X-2(+) was signifycantly higher than that in both Survivin(-) and COX-2(-). It was speculated that there may be so-me uncertain mechanism of coordination or interface regul-ation in accelerating tumor angiogenesis.4 At the same time this experimental study prompted the target to Survivin, the COX-2 gene antitumor treatmentmay provide theory basis for antibloodvessel production tr-eatment of the meningioma.