Induction Of Cytokine On Hepatocytes By Cytokine Inducer PPD, PolyI: C And PHA

ObjectiveTo investgate if cytokines can be Induced by cytokine Inducer in hepatocytes in vitro.MethodsHepG2 and 2.2.15 Cells were treated with maximal tolerance dosage of polyI:C ,PHA, and PPD. After 24h and 48h ,the contents of IFN-α,TNF-αand IFN-γboth in the supernatant and cell were determined by ELISA, and the contents of HBV virus marker sAg,eAg in 2.2.15 Cells in the supernatant and cell were also determined by ELISA.Results1,IFN-αwere detected in the supernatant and lysate in 24h and 48h,IFN-αlevels were significantly higher than that of control groups,IFN-αlevels in 24h were significantly higher than in 48h,IFN-αlevels in intra-cell and ecto-cell were significant difference。HepG2 were treated with PPD,polyI:C and PHA, TNF-αwere detected in the supernatant and lysate, The difference of TNF-αlevels were not significant with control groups,The difference of IFN-αlevels in intra-cell and ecto-cell were not significant. The difference of IFN-αlevels in 24h and 48h were not significant. HepG2 were treated with PPD,polyI:C and PHA , IFN-γwere detected in the supernatant ,The difference of IFN-γlevels were not significant with control groups,HepG2 were treated with PPD and PHA,IFN-γwere detected in the lysate,The difference of IFN-γlevels were not significant with control groups,The difference of IFN-γlevels in 24h and 48h were not significant.2. 2.2.15cells were treated with PPD,polyI:C and PHA with the maximal tolerance dosage,IFN-αwere detected in the supernatant and lysate,The difference of IFN-αlevels were significant with control groups,The content of IFN-αin intra-cell were higher than in ecto-cell,the difference was significant. The difference in 24h and 48h was not significant .IFN-γ,TFN-αwere detected in the supernatant and lysate,The difference was not significant with control groups,The difference in ecto-cell and intra-cell was not significant . The difference in 24h and 48hwas not significant.3.2.2.15cells were treated with PPD,polyI:C and PHA with the maximal tolerance dosage,the effect of cleaning HbsAg and HBeAg was not significant with control groups.Conclusion1. IFN-αcan be Induced by PPD,polyI:C and PHA in HepG2 cell and then be secreted out;The content of IFN-αand action time were direct ratio. IFN-γcan be induced by PPD and PHA and then were not secreted out; The content of IFN-γand action time were independence. IFN-γcan be induced by polyI:C ,but the effect was not obviously. TNF-αcan be Induced byPPD,polyI:C and PHA but the effect was not obviously.2. IFN-αcan be Induced by PPD,polyI:C and PHA in2.2.15 cell and then be secreted out ; The content of IFN-αand action time were independence. IFN-γ,TNF-αcan be induced by PPD,polyI:C and PHA but the effect was not obviously3. 2.2.15 cells were treated with PPD,polyI:C and PHA,the effect of cleaning sAg and eAg was not significant.