The Expression Of Ubiquitin In Different Liver Tissues With Chronic Hepatitis

OBJECTIVE: The main function of ubiquitin-proteasome system is degradation of the intracellular proteic substance. The ubiquitin-proteasomes systems play magnificent roles in maintenance of many important physiological function in cells. The functional disorder of UPS is associated with several heritage and acquired diseases. The aim of this study was to investigate ubiquitin expression levels of different liver tissues which are grouped by inflammation scores with chronic hepatitis B, the relationship with pathematology change,the effect of invasion mechanism.METHODS: A total of 57 patients were studies. The patients were divided into four groups according to the necroinflammation scores. After hematoxylin-eosin(HE),Gormori dye, pathologic grading,staging,fibrosis scores and immunohistochemistry stain,scores of semi-quantitative scoring system (SSS) were evaluated, ubiquitin in liver tissue were examined by immuno-histochemical techniques.RESULTS: Ubiquitin is mainly expressed on the liver karyon,the color is brown. There is no statistical difference between G1 group and G2 group (P>0.05) . Compared G1 group to G3 group and G2 group to G3group ,there is statistical difference (P<0.05). Compared G1 group to G4 group and G2 group to G4 group ,there is statistical difference (P<0.05). There is statistical difference between G3 group and G4 group (P<0.05).CONCLUSION: With the methods of inflammation scores in all patients , there was statistical difference between G1,G2 and G3 , G1,G2 and G4 , G3 and G4 in showing the expression of ubiquitin .Tt shows that affected ubiquitin lowers degradation of the intracellular proteic substance by some kinds of mechanism .In this way ,the aggravation of liver cells happened.