| Objective:To establish rat models of Steroid-Avascular Necrosis of Femoral Head,Observe the effects of Tao Hong Si Wu Decoction on genetic expression of transforming growth.factor-β1(TGF-β1).To interpret the mechanism of the effect on Steroid-Avascular Necrosis of Femoral Head by activating blood circulation,and offer a effective method to clinical.Method:cleaner-40 SD rats,half male and half female,weight 200±20g,were randomly divided into 2 groups:4 rats were in common group and 36 rats were in experimental group.The rats in common group were as normal control;the rats in experimental group were administered with hydroxyprednisone through gluteus injection(teice a week)for 6 weeks as control;there were 4 rats were killed in each group after 6 weeks,to be assure that the model were succed.All surplus rats were divided into healing group and control group:the healing group were administrated with tao hong si wu decoction 12.3ml/kg per day,the control group were administrated with sodium chloride 12.3ml/kg per day.then,ater 6 and 8 weeks,killed the animal,and detected all indexes.Results:1.The administration of large dose glucocorticoid induced the weight of the model group rats decreased gradually from the baseline in short time,and after intragastric administration,the weight of the treat group rats increased significantly than the control group. (P＜0.05) 2.Histopathological study:Hormones can model group vacancies bone lacunae significantly increased,Trabecular bone fine reduced,Marrow fat cells increase,and more significantly than the blank group.3.electron microscope analysis showed osteocytes and osteoblasts in model group decreased and osteocytes had degeneration signs or fatty drips appeared inside osteocytes,and intramedullary lipocytes became largerand more.4.local blood vessel microdensity in femoral head:the treat group blood vessel microdensity was higher than control group,and the two group had significant statistical differences.5.the expression of TGF-βby immunohistochemistry and image analysis:treat group the expression of Femoral Head TGF-βincreased significantly than control group and two group had significant differences.6,serum alkaline phosphatase levels in:control group,serum alkaline phosphatase levels increased,compared with the control group,there were significant differences(P＜0.01).7,serum levels of TGF-β1:serum in the treatment group Expression of TGF-β1 increased,compared with the control group had significant difference(P＜0.01).8, the femoral head local TGF-β1 mRNA transcription:treatment group in the first six weeks, expressed that the increase in the first eight weeks,expressed also reduced,and the control group in the first six weeks,expressed a decrease in the first eight weeks,was not detected to TGF-β1 mRNA expression of difference between the two groups was significant(p＜0.01). TGF-β1 mRNA in serum and the femoral head with local expression of TGF-β1 in consistency.Conclusion:1.rat abdominal cavity prednisolone acetate injection plus intermittent standing avascular necrosis modeling stability,good repeatability.2.Taohongsiwu through the promotion of hormone-ischemic necrosis of the femoral head rat model of TGF-β1 mRNA transcription,and promote expression of TGF-β1.3.Taohongsiwu soup can promote hormone avascular necrosis of the rat model of the expression of TGF-β1,which can effectively promote local femoral head microvascular regeneration.4.Taohongsiwu soup can not unly confront the role of hormones,but also antagonism bone destruction caused by hormone and lower serum ALP content.
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