Empirical Study On The Effects Of High Glucose And The Intervene Of Tea Polyphenols On The Rat Lens Epithelium

Objective To observe ROS expression in mitochondria and endoplasmic reticulum of rat lens epithelium cultured in high-glucose and the changes of them after the intervention of TP. To study the causes and effects of these changes, and to further explore the pathogenesis of diabetic cataract and TP's curative effect.Methods 1 Rat lens epithelium cells were cultured in vitro in different glucose concentration to simulate the hyperglycaemia in vivo. The morphous changes of the cells were then observed when they were intervened in different concentration of TP. 2 ROS changes in mitochondria of rat lens epithelium cells in each condition were detected with scanning laser confocal microscope to analyze the effects of high glucose and TP on ROS expression of cells mitochondria. 3 The mitochondria membrane potential (MMP) and cell death rate (CDR) were measured with flow cytometer to analyze the effects of high glucose and TP on MMP and CDR. 4 The expression of endocytoplasmic reticulum in rat lens epithelium was detected with immunocytochemistry and immunofluorescence to analyze the effects of high glucose and TP on endocytoplasmic reticulum.Results 1 Rat lens epithelium cells cultured in high-glucose medium lost their inherent characteristics and presented diversity, irregularity and inhomogeneity. The cells were gradually swelling, emerging vacuole and granula, decreasing, becoming rarefacive and aging easily after passage. Whereas, the cells interfered with TP retained better morphous, adjoined and engaged with each other closely, arranged regularly and emerged less swelling, vacuole and granula. There were no decreasing and aging after passage of the cells also. 2 In high-glucose, ROS generated from the lens epithelium cells increased. Inversely, it decreased in groups interfered with TP. 3 In high-glucose, CDR increased obviously and MMP decreased. It showed reverse outcomes in groups interfered with TP. 4 The positive expression of endocytoplasmic reticulum in rat lens epithelium decreased in high glucose. On the contrary, the expression increased in groups interfered with TP.Conclusions High glucose can be triggered by various means lens epithelial cell death, the death of epithelial cells may be diabetic cataract in one of the reasons, and tea polyphenols may inhibit the formation of mitochondrial reactive oxygen species can be protected from damage to lens epithelial cells and maintaining its normal structure and function, which may delay the occurrence of diabetic cataract, tea polyphenols may become effective prevention and treatment of diabetic cataract of new drugs for the large number of patients with diabetes promising.