Effects Of Prostaglandin E₁ And Budesonide Combined Terbutaline In Neonatal Rats With Hyperoxia Induced Lung Injury

Objective:To investigate the effects of Prostaglandin E₁(PGE₁) and Budesonide combined Terbutaline in neonatal rats with hyperoxia induced lung injury.Methods:A total 120 term neonatal Wistar rats were randomly divided in to air,model, Budesonide combined Terbutaline and PGE₁ treated groups(every group n=30).The air group was exposed to room air,and other tree groups were exposed 90%O₂ for 14 days.The treatment groups received Budesonide combined Terbutaline and PGE₁ by nebulization after exposed 90%O₂ 2 days,the air and model groups received normal saline by nebulization.At each time interval of 3,7 and 14 days after experiment,ten rats of each group were sacrificed.The levels of MDA and TP were determined from bronchoalveolar lavage fluid(BALF),pathological changes of lung were observed under a light microscope.The expression of CTGF and IFN-γand TNF-αwere detected by immunohistochemistry.Results:The expression level of MDA and TP was less in Budesonide combined Terbutaline group(0.76±0.04,0.45±0.02) and PGE₁(0.85±0.04,0.44±0.02) group than in the model group(2.14±0.11,0.55±0.02).The expression strength of CTGF and IFN-γand TNF-αwas less in Budesonide combined Terbutaline group(19.85,20.50,20.61) and PGE₁(21.50,21.60, 21.50) group than in the model group(35.50,36.15,35.50).The level of MDA and TP from BALF was significantly decreased,the expression strength of CTGF and IFN-γand TNF-αwas significantly lower in the treatment groups than that the model group;there were all significant difference between the treatment groups and model group(All P <0.05),but there was no significant difference between Budesonide combined Terbutaline and PGE₁(P >0.05).Conclusion:The Budesonide combined Terbutaline and PGE₁ had protective effect in neonatal rats with hyperoxia-induced lung injury.This may contribute to one of possible mechanism that inhibit to release the oxygen free radical and cytokines.