Studies On The Determination Of Nitroimidazoles And The Interaction Between Them And Serum Albumin By Electrochemical Methods

Chapter 1:5-Nitroimidazoles had been widely used in animal drugs and human clinical medicine,the carcinogensis and mutability of nitroimidazoles damage human health because of its residual drugs,physicochemical property and analytical method research progressivity of nitroimidazolesh have been introduced in this chapter.Scientists are interesting in study of interactivity between drug and serum albumin in present,in this chapter, interactivity between drug and serum albumin has been reviewed.Superiority of electrochemical methodology is developed about method,technology and application widely.The electrochemical methods and its application have been introduced in this chapter.Compared with normal electrode,chemical modified electrode can strengthen electrochemical signal of detected materials,carbon nanotube modified electrodes are attention specially by electrochemical workers.In this chapter,study of chemical modified electrode and carbon nanotube modified electrodes has been introduced.Chapter 2:The electrochemical behavior and determination of metronidazole on the silver disk electrode have been studied in this chapter, metronidazole tablets have been detected,the results showed that this method can be used for the determination and pharmacokinetics study of metronidazole.The current property of reduction peak of metronidazole was investigated by the cyclic voltammetry.The characteristic irreversible adsorption of the peak was proved.Chapter 3:Electrodes based on carbon nanotubes are an attractive research area now.Carbon nanotubes display remarkable nanostructures in that they combine large surface area,excellent electrical conductivity,good chemical stability and significant mechanical strength.The subtle electronic properties suggest that carbon nanotubes have the ability to promote electron-transfer reactions when used as an electrode material in electrochemical reactions,which represents a new area of application for carbon nanotubes.In this study,the MWNTs were dropped onto the surface of a glassy carbon(GC) electrode to form an MWNT-modified one.The electrochemical behavior of ornidazole at such a modified electrode was investigated in detail,a reduction peak was obtained in ornidazole solution at the MWNT-modified electrode,compared with a bare electrode,the peak current had obviously increased.Chapter 4:Study on the interaction drugs and serum albumin help clarify drugs save and operation process,at the same time,it can provide valuable information regarding drug design,development and pharmacology research.In this chapter,the electrochemical methods are used for study on the interaction between metronidazole and bovine serum albumin.The results showed that a sensitive reduction peak of metronidazole has been obtained, the peak potential was-0.57V(vs.SCE).But bovine serum albumin has no reduction peak in the scanning area of-0.3～-0.8V.When bovine serum albumin was added in metronidazole solution,the reduction peak of metronidazole will fall down,the peak current declining value was directly proportional to the bovine serum albumin concentration.In addition,the interaction mechanism between metronidazole and BSA has been studied,the results demonstrate that combination of them mainly by hydrophobicity.