Research On The Chemotherapeutic Sensitivity Of Cerebral Gliomas In Vitro

Objective: To compare the effect of 5 antineoplastic agents(VM-26, BCNU, CDDP, VCR, MTX) on suppressing cerebral glioma cells in vitro with colorimetric MTT assay, and investigate the relationship of tumor pathological type, sex, age and Karnofsky Performance Status (KPS) with the sensitivity of chemotherapeutic drugs, so as to supply some instructions for selecting chemotherapeutic plan for individual patient.Methods: 50 neurosurgical biopsies were plated in 96-well tissue culture plates and incubated for 72h with 5 different antineoplastic agents, and 3 different concentrations of each agent were tested. The sensitivity of five chemotherapeutic drugs combined administration or solo- administration to brain glioma cells from 50 fresh specimens was measured by utilizing MTT colorimetric assay.Results: To carry on the drug sensitivity in vitro for 50 brain glioma specimens, 42 drug sensitivity results were acquired, and the success rate of which reached to 84%. In 42 cases, the sensitivity sequence of five chemotherapeutic drugs from high to low was VM-26, BCNU, CDDP, VCR, MTX. The inhibition rate of combined chemotherapy (BCNU+VM-26+CDDP) or (BCNU+VCR+MTX) against glioma was higher obviously than five respectively. Patient age, sex did not prove to be significant correlated with the most agents chemosensitivity in vitro by statistics. Except VM-26,Karnofsky Performance Status (KPS) was not correlated with the drugs chemosensitivity . Tumor pathological type was correlated with chemosentivity of the agents.Conclusion: The MTT colorimetric assay is a rapid method of screening antineoplastic agents against gliomas for cytotoxicity. Combined chemotherapy of VM-26, BCNU and CDDP is obviously effective to glioma cells and it provides higher inhibition rate and lower dosage. Tumor pathological type was correlated with chemosentivity of the agents.