Study On The Quality Of Folium Nelumbinis In Hubei Province And The Evaluation Of The Activity Of The Alkaloids

Folium Nelumbinis is the dried leaf of Nelumbo nucifera Gaertn. It has the effect of clearing away summer-heat and heat evil, ascending the lucid yang and cooling blood to stop bleeding. It grows in middle and south of China and the region of Zhejiang and jiangsu. especially in Hubei province, it has a long history of cultivation and abundance resource. Folium Nelumbinis contains kinds of chemical ingredients and is added to single and compound recipes treating obesity clinically.Because of the potential clinical value of Folium Nelumbinis and the extensive resource, its quality is difficult to control. Being lack of unified estimated standard causes much inconvenience in clinical application. The thesis aims to establish methods with HPLC and acid dye colorimetry to determine the content of nuciferine, quercetin and total alkaloids in Folium Nelumbinis respectively. The results show that the contents of index ingredients in areas of Hanchuan, Qianjiang and Hanyang were relatively higher than that from other habitats and the month of September is the best time to collect.On the basis of confirming the index ingredients, the alkaloids were extracted with macroporous resins from Folium Nelumbinis. The process was optimized by employing orthogonal test design with the content of nuciferine as the index. The resin AB-8 had much better adsorption capacity and desorption ratio by using 8 BV 40% aqueous ethanol to extract the alkaloids in Folium Nelumbinis than other resins. The results showed that AB-8 possessed the highest absorption capacity under the concentration of sample 0.1g?mL-1, the condition of flowing rate of 1.0mL?min-1. Macroporous Resin AB-8 may be value of isolating and purifying the alkaloids from Folium Nelumbinis.The alkaloids extract was prepared according to the technological process above, and the effect on the trophism obesity rat and blood-fat index was investigated. The result showed that the rats'weight, Lee'indexes, and the weight of rats'fat was obviously descended. It also can reduced TC and TG, yet improve the HDL-C/LDL-C in blood serum. Thus it can be seen that the alkaloids extract of Folium Nelumbinis have significant effect on the reduction of fat and blood lipids.Generally speaking, through the analysis of alkaloids and flavones in Hubei province and optimized method of extraction of alkaloids with macroporous adsorptive resins, it supplied the essential precondition to evaluate the activity of anti-obesity and degrading the blood-fat with Folium Nelumbinis. Simultaneously it is possible to elucidate its molecule mechanism with concerned experimental data. It established basis to set up the system of quality evaluation combining the chemistry and biology index. It is in favor of the promote of Folium Nelumbinis economy in Hubei province. It could develop the novel nutritious food and drinks, which will increase the income of peasants who plant Folium Nelumbinis.