Study On Dose-effect Relationship Of The Microsecond Level Steep Pulsed Treatment To Tumor

Tumor has already become the main disease for our health, the tradition treatment means, such as operation treatment, tadiotherapy, chemical medication, had considerable development, but all of them have certain action such as adaptation disease, contraindication and side effect. Steep pulsed, acting as a new kind of treatment techmique to tumor, is of minimally invasive, innocuity side effect merit and so on, it shows a good development prospect for future and stirs up research upsurge in international-wide.This paper is studied on the normal and tumor tissue of rabbit's liver, adopted the method of the combination of animal experiment and electric field simulation, researched the threshold and the dose-effect of the irreversibility electrical breakdown (IREB) under steep pulsed.Firstly, physical model of the normal and tumor tissue have been established, their electrical field distributions of steep pulsed have been simulated and experimented by FEMLAB software. Finding: The results of simulation and experiment have anastomosed each other. Feasibility can be confirmed that we can adopt the emulation method to research the bioelectricity effect about steep pulsed treat tumor. For different electrode array of electrical field distribution, it is simulated by FEMLAB software. It's discovered that electrode diameter, the distance between electrodes and the number of electrode have a certain influence on the electrical field distribution, so it needs to design different electrode array, according to different clinic demands.Secondly, the object of study is the normal tissue of the rabbit's liver. Adopt the method of the combination the FEMLAB simulation and TTC dying, the electric-field intensity threshold of the IREB is 926±30V/cm. Labeled by Bleomycin and observed the extent of the chromatin concentration, we can draw the conclusion that the electric-field intensity threshold of the reversibility electrical breakdown (REB) is 807±30V/cm. We analysis the electric-field intensity threshold of electrical breakdown from standpoint of the transmembrane voltage. Through study the influence of electrical parameter on dose-effect relationship, such as peak voltage, pulse width, frequency, we can draw a conclusion that the putrescence area of the rabbit's liver organization would increase as the dose of the steep pulsed, but dose-effect relationship subsist saturation tendency. Finally, the object of study is the VX2 tumor tissue of the rabbit's liver. Adopt the method of the combination the FEMLAB simulation and animal experiment, the electric-field intensity threshold of the IREB of the rabbit's VX2 tumor cell is 838±46V/cm. By TTC dying and measure the steep pulsed of different peak voltage, we can observe and compare the back and forth volume between VX2 tumor tissue and putrescence tissue: the putrescence volume of VX2 tumor tissue is increasing as the peak voltage of the steep pulsed increases, the level of growth about tumor decreases, it is distinct dose-effect relationship (p<0.05). We have counted the transmembrane voltage of the normal tissue and tumor tissue, finding: the transmembrane voltage of the tumor is higher than the normal. So the damnification of the tumor is easier than the normal, the selectivity of the kill-effect of the steep pulsed have been showed.