Effect Of Pulsed Magnetic Fields On The Single Neuron Ion Channel

With the development of the modern industry, electromagnetic fields has become more extensive effect on human body, but the effects has not been clarified. Many magnetic healing instruments have come out, and some have already applied in clinic, but its mechanism has not been identified. So the study of magnetic fields effect on cells has important realistic significance.In this paper, we examined the effects of extremely low frequency low density pulsed electromagnetic fields on Na+ and K+ voltage-gated ion channel of rat cortical neurons at the first time. Pulsed electromagnetic fields that frequency is 15Hz and the intensity is 1.4mT was imposed on the acutely isolated rat cortical neurons, and properties of K+ and Na+ voltage-gated ion channel were surveyed with the whole-cell patch clamp technique. The results show that pulsed electromagnetic fields can change the action potentials and current amplitudes of the ion channel. The amplitudes were reversibly changed in a voltage-dependent manner. The maximum amplitude of INa current was reduced and also the activation and inactivation process are influenced by pulsed electromagnetic fields'exposure.Thus,Depolarization and Hyperpolarization process of action potential was affected.And further physiological functions of neurons were altered as a result of the exposure.The amplitude currents of IA and IK was obviously inhibited and the activation and inactivation process of K+ channel significantly affected by the pulsed electromagnetic fields'exposure, this can change the properties of Transient Outward Potassium channel . So physiological functions of neurons are adjusted, which might contribute to the restoration of damaged neuron.It is a new method to research the low density extremely low frequency pulsed electromagnetic fields biological effect on cell and molecule level with whole patch-clamp technique, this paper study the effect of pulsed magnetic fields on the cortical neurons, it was provided as a recording to completely and deeply studying the electromagnetic fields'biological effect.