| ObjectivObjective: This paper is to study the expression of PTEN and Survivin innormal endometrium, endometrial hyperplasia, atypical endometrial hyperplasiaand endometrial cancer tissue, and explore the relationship of PTEN and Survivinwith endometrial carcinoma, clinical stage, histological class, as well as themutual relationship between PTEN and Survivin in tumor development andtransfer.Methods: Randomly selected 20 cases of Normal endometrial tissueconfirmed by by pathology afer gynecologic surgery, 20 cases of endometrialgyperplasia tissue,atypical Endometrial hyperplasia tissue, 40 cases of Endometrial adenocarcinomatissue. All of the subjects operated from March 2007 to November 2008. Patientsare aged from 45 to 72 years old, and the average age is 50 years old. They didnot accept preoperative radiotherapy and chemotherapy, and did not merge inother malignant tumor. The specimens of endometrial cancer are alladenocarcinoma. In accordance with the histological grading criteria of the WHO(World Health Organization): 20 cases of well-differentiated group, 17 cases ofdifferentiated group, three cases of poorly differentiated group. Stage the patientswith endometrial cancer according to established criteria for surgical staging ofFIGO (International Obstetrics Union), and all specimens are from stage I, II, IIIwith no IV. Specimens are fixed by 10% formalin, embedded by conventionalparaffin. Cut serial sections of 4μm thick, and each case with three slices. Thethree slices are processed by HE staining, PTEN immunohistochemical staining,and Survivin immunohistochemical staining. Known positive slice in the kit are as a positive control. All the slices were identified by pathology experts. Stain thetissues by SP method to detect PTEN and Survivin expression.ResultResults: 1. PTEN protein mainly localized in the cytoplasm, occasionally inthe nucleus, The expression rates of PTEN protein in the normal endometrium,endometrial hyperplasia, atypical endometrial hyperplasia, endometrial cancertissue are 80%, 75%, 60%, 30% perspectively. The expression of PTEN proteinin normal endometrial tissue and in proliferative phase endometrium wassignificantly lower than that in endometrial atypical hyperplasia and endometrialadenocarcinoma tissue (P <0.05), and there are no significant difference of theexpression between normal endometrial tissue and proliferative phaseendometrium (P> 0.05). There are no significant difference of the expressionbetween atypical proliferative endometrial carcinoma and endometrial carcinoma(P> 0.05).2.Survivin protein mainly localized in the cytoplasm, nucleus have a smallamount of expression. The expression rates of Survivin protein in the normalendometrium, endometrial hyperplasia, atypical endometrial hyperplasia,endometrial cancer tissue are 15%, 20%, 50%, 80% perspectively. Theexpression of Survivin protein in normal endometrial tissue and in proliferativephase endometrium was significantly higher than that in endometrial atypicalhyperplasia and endometrial adenocarcinoma tissue (P <0.05), and there are nosignificant difference of the expression between normal endometrial tissue andproliferative phase endometrium (P> 0.05). There is statistical difference of theexpression between atypical proliferative endometrial carcinoma and endometrialcarcinoma (P< 0.05).3. The positive expression rates of PTEN protein in well-differentiatedendometrial cancer group, the differentiation group of endometrial carcinoma,poorly differentiated group of endometrial cancer are of 30%, 30%, 33%respectively. There is no significant statistical difference (P> 0.05) between eachtwo groups; In the surgical pathological staging, the positive expression rates of Phase I Group, II Phase Group, III Phase Group are 34%, 25%, 17% respectively,There is no significant statistical difference (P> 0.05) between each two groups.4. The positive expression rates of Survivin protein in well-differentiatedendometrial cancer group, the differentiation group of endometrial carcinoma,poorly differentiated group of endometrial cancer are of 65%, 94%, 100%respectively. There is no significant statistical difference (P> 0.05) between eachtwo groups; In the surgical pathological staging, the positive expression rates ofPhase I Group, II Phase Group, III Phase Group are 65%, 82%, 100%respectively, There is no significant statistical difference (P> 0.05) between eachtwo groups.5. In the normal endometrium group PTEN protein expression is positiveand at the same time the negative expression of Survivin protein takes up 75%,accounting for obvious advantage. In the Proliferative phase endometrium groupPTEN protein expression is positive and at the same time the negative expressionof Survivin protein takes up 70%, accounting for obvious advantage. In theatypical Proliferative phase endometrium group, there is no significant differencebetween PTEN protein expression and Survivin protein expression. Theexpression of PTEN protein is negative while the survivin protein positiveexpression takes up 65% , accounting for obvious advantage in the group.ConclusioConclusion: 1. The expression rates of PTEN gene in the normalendometrium, endometrial hyperplasia, atypical endometrial hyperplasia,endometrial cancer tissue are gradually weakening. The expression of PTEN inthe normal endometrium and endometrial hyperplasia is significantly higher thanthat of atypical endometrial hyperplasia and endometrial cancer. The expressionof PTEN gene is related to the Pathogenesis of endometrial cancer.2. The expression of PTEN gene in endometrial cancer has no significantrelation to histological grade and pathological staging surgery. The expression ofPTEN gene has no significant relation to pathological features.3. The expression rates of suvivin gene in the normal endometrium, endometrial hyperplasia, atypical endometrial hyperplasia, endometrial cancertissue are gradually increasing. The expression of Survivin in the normalendometrium and endometrial hyperplasia is significantly lower than that ofatypical endometrial hyperplasia and endometrial cancer. There is statisticaldifference between the atypical endometrial hyperplasia expression andendometrial cancer expression. The expression of Survivin gene is related to thePathogenesis and disease progression of endometrial cancer.4. There is no significant correlation of Survivin expression in endometrialcarcinoma and histological grade, pathological staging surgery. There was no nosignificant correlation between Survivin gene expression and the pathologicalfeatures of endometrial cancer.5. PTEN and Survivin genes are both involved in the pathogenesis ofendometrial cancer, but the two have opposite biological effects, and there isnegative correlation. Combining the two, the joint detection can provide a certainguide for the accurate diagnosis of endometrial cancer and subsequent genetherapy. |
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