| Chemotherapy in the treatment of choriocarcinoma has a decisive position, in which MTX is the drug of choice for the treatment of choriocarcinoma, but resistance was serious, therefore, reversed down the resistance of cancer cells and restore their sensitivity to MTX is currently the research focus. Screening of traditional Chinese medicine in recent years from a high efficiency, low toxicity, multi-agent target for the reversal of a new research focus. Studies have shown that can Shikonin JAR choriocarcinoma cells sensitive to apoptosis, reversal of drug-resistant choriocarcinoma cells JAR / MTX to MTX resistance. As a result of MRP and LRP is found in recent years, resistance-associated genes, in the multi-drug resistance mechanisms play an important role, then Shikonin of JAR / MTX-resistant cells with reversal of the role of MRP and LRP expression, it is not clear. Therefore, this study by observing Shikonin of JAR / MTX cell growth inhibition and the role of resistance and at the same time to observe its JAR / MTX cells, MRP and LRP expression. Shikonin preliminary study of the JAR / MTX cells, a mechanism of resistance in order to better guide clinical treatment of choriocarcinoma Shikonin resistance. Objective:Discussion Shikonin of JAR / MTX-resistant cells and the reversal of the role of MRP and LRP expression. Methods : Experiment divided into 4 groups:â‘ control group,â‘¡drug-resistant cells+Chinese medicines: JAR/MTX+shikonin (concentration 0.2ug/ml),â‘¢drug-resistant cells+chemotherapy group: JAR/MTX+MTX(concentration 2ug/ml),â‘£drug-resistant cells+ Chinese medicines+chemotherapy group: JAR/MTX+MTX (concentration 2ug/ml) + shikonin (concentration 0.2ug/ml). MTT assay using cells in each group 24 hours after administration of cell death, respectively, using RT-PCR and flow cytometry detection of cells in each group 24 hours after medication MRP and LRP expression of mRNA and protein changes. Results:1.Shikonin of JAR / MTX cell growth inhibition and the role of resistance.This test showed that compared with the MTX group, group Shikonin JAR / MTX cells, increased mortality, with statistically significant differences (P <0.05), that the treatment used alone Shikonin of JAR / MTX cells with growth inhibition , but its role in the relatively weak; Shikonin when combined with MTX, the cell death rate compared with the MTX group a significant increase in the difference was significant statistical significance (P <0.01), that can enhance MTX Shikonin of JAR / MTX cell growth inhibition, with the role of resistance, the resistance multiplier of 5.3. This shows that shikonin has strong anti-tumor activity against a variety of tumor cells can also be sensitized and drug-resistant reverse the role of chemotherapy has the potential to become an important phenomenon of drug resistance and reducing remedies side effects of chemotherapy.2. Shikonin of JAR / MTX cells, MRP and LRP expression of the mRNA.This test showed that the control group, MTX group, shikonin and shikonin + MTX group 24 hours after medication, were seen positive for MRP and LRP gene expression Shikonin MRP and LRP group with the control group the expression of MRP and weakened compared to the expression of LRP, with a statistically significant difference (P <0.05); Shikonin + MTX group of MRP and LRP expression with the control group, MRP and LRP expression significantly decreased compared, the difference was significant statistical significance (P <0.01).3. Shikonin of JAR / MTX cells of MRP and LRP protein expression.This test showed that group Shikonin MRP and LRP protein expression levels, with the control group, MRP and LRP protein expression level differences with statistical significance (P <0.05); and + MTX group Shikonin MRP and LRP the level of protein expression decreased significantly with the control group, MRP and LRP protein expression level of the difference was significant compared to statistical significance (P <0.01).Whether shikonin alone or with MTX Shikonin combination, JAR / MTX cells, MRP and LRP expression levels of mRNA and protein expression decreased the level of that in MRP and LRP choriocarcinoma as an index to study multi-drug resistance either from the gene level or protein level, there are very good consistency, but there are other genes involved in regulating protein expression of resistance requires further experiments to prove.Conclusions:1. Shikonin in vitro of JAR / MTX cells, growth inhibition has a direct.2. Shikonin MTX in vitro can be enhanced on the JAR / MTX cell growth inhibition, restoration of JAR / MTX cells to MTX sensitivity, with sensitivity and resistance by the role reversal.3. Shikonin sensitizer and the mechanisms of resistance may be related to JAR / MTX cells decreased the expression of the MRP.4. Shikonin sensitizer and the mechanisms of resistance may be related to JAR / MTX cells decreased the expression of the LRP.In short, not only for Shikonin JAR / MTX cells, growth has a direct inhibitory effect of MTX at the same time enhance the JAR / MTX cell growth inhibition, the restoration of multi-drug-resistant choriocarcinoma cells to MTX sensitivity and sensitization with the role of resistance, its mechanism may be related to the expression of MRP and LRP decline. Therefore, shikonin may become the future clinical treatment of chemotherapy-resistant choriocarcinoma important phenomenon remedial measures, while reducing side effects of chemotherapy and improve the quality of life of patients and the prognosis for drug-resistant choriocarcinoma chemotherapy to provide a new therapeutic approach.
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