Study On The Hypnogenic Effect And Mechanism Of Moxibustion With Seed-sized Moxa Cone In Different Quantities

Objective:1.To observe the effect of moxibustion with seed-sized moxa cone on sleep time at different quantities in sodium pentobarbital rats.2.To compare the effect of amino acid neurotransmitters in brain,Glutamatc(Glu),γ-aminobutyric acid(GABA), Nitrous oxide(NO) and Nitricoxide synthase(NOS) by moxibustion with seed-sized moxa cone in insomnia rats caused by DL-P-Chlorophenylalanine(PCPA) hypothalamus at different quantities.3.To explore the mechanism of moxibustion with seed-sized moxa cone in different quantities on insomnia.Methods:1.Twenty-four rats were randomly divided into four groups,control group, seed-sized moxibustion groupⅠ(three moxa-cone),seed-sized moxibustion groupⅡ(five moxa-cone) and seed-sized moxibustion groupⅢ(ten moxa-cone).The different sleep time in these four groups were observed by the method of prolonging sleep time of rat made by sodium pentobarbital.2.Thirty-six wistar rats were randomly divided into six groups:control group(n=6),model group(n=6),diazepam group(n=6),seed-sized moxibustion groupⅠ(n=6),seed-sized moxibustion groupⅡ(n=6),seed-sized moxibustion groupⅢ(n=6).PCPA abdominal injection rats were used as the models of insomnia in the experiments.Amino acid neurotransmitters such as Glu,GABA,NO and NOS in the hypothalamus of insomnia rats were observed by Ion Chromatograph and Nitrate Reductase Assay.Results:1.Compared with control group,the sleep time of Pentobarbital sodium rats was obviously prolonged in three seed-sized moxibustion treatment groups.2.After PCPA abdominal injection the normal sleep cycle of rats disappeared.Both of seed-sized moxibustion groups and diazepam group could recover the sleep cycle of insomnia rats caused by PCPA.3.After PCPA abdominal injection the content of GABA in the hypothalamus of rats obviously decreased with comparison to control group,and the content of Glu,NO,NOS in the hypothalamus of rats obviously increased with comparison to control group.4.Diazepam has no effect on the content of Glu,GABA,NO and NOS in rats' hypothalamus.5.After the grain-sized moxibustion treatment,the content of GABA increased in contrast with those of model group,while that of Glu,NO,NOS decreased in contrast with those of control group.6.In these three grain-sized moxibustion groups, groupⅠhas a better effect than groupⅡandⅢ.Results:1.Moxibustion with seed-sized moxa cone therapy can prolong the sleep time, keeping Insomnia rats calm and hypnogogic.2.Moxibustion with seed-sized moxa cone therapy is an effective method in the treatment of insomnia,and is superior to diazepam. The study also indicates that the mechanism of treating insomnia by moxibuxion maybe increase the content of GABA and decrease the content of Glu,NO and NOS.3.The effect of treating insomnia rats caused by PCPA by seed-sized moxibustion three moxa-cone maybe better than that of seed-sized moxibustion five moxa-cone.This provides objective theoretical evidence to the clinical treatment of insomnia.