Research On Synergism And Attenuation Effect Of Taurine On Cyclophosphamide

Objective:To investigate synergism and attenuation effect of taurine on CTX.Methods: Fifty Kim Ming healthy mice were given 0.2ml of H₂₂ tumor cell suspension(containing 2×10⁶ cells-ml^-1)at the mice subaxillary region. The mice were randomly divided into five groups: model control group; CTX control group and three trial groups. The model group was given an intraperitoneal injection of sodium chloride; the CTX group was given CTX 20mg·kg^-1; and the three trial groups were given CTX+Tau (20+50) mg·kg^-1,(20+100) mg·kg^-1and (20+200) mg·kg^-1 respectively, q.d. for eight days. The inhibition rate of CTX+Tau and CTX on H₂₂ tumor, the spleen index and the thymus index were observed; The levels of white blood cells(WBC) and bone marrow nucleate cells were measured; The activities of NK cells and lymphocyte proliferation were analyzed by MTT. S₁₈₀ was studied in the same manner as H₂₂.Results: Model of H₂₂ tumor:â‘ The tumor inhibition rate of the CTX group and the three trial groups were 50.32%, 58.06%, 67.74%, and 72.26% respectively.â‘¡Compared with the model group, the bone marrow nucleate cells and WBC's levels of the CTX group decreased significantly (P<0.01,P<0.01);Compared with the CTX group, the bone marrow cells and WBC's levels of the Tau +CTX groups increased (P<0.05, P<0.01).â‘¢Compared with the model group, spleen index and thymus index of the CTX group decreased (P<0.05, P<0.01), while compared with the CTX group, spleen index and thymus index of the Tau+CTX groups were improved and the difference was significant (P<0.05, P<0.01).â‘£Compared with the model group, NK cell activity of the CTX group decreased (P<0.05);NK cell activity of Tau+CTX groups(the mudium,high-dose groups)were higher than the CTX group(P<0.05, P<0.01).⑤Compared with the model group, T and B lymphocyte proliferation activity of the CTX group had no obvious difference; Compared with the CTX group, T and B lymphocyte proliferation activity of the Tau+CTX groups increased (P<0.05, P<0.01). Model of S₁₈₀ tumor:â‘ The tumor inhibition rate of the CTX group and the three trial groups were 56.65%, 60.10%, 72.41%, and 86.70% respectively.â‘¡Compared with the model group, the bone marrow nucleate cells and WBC's levels of the CTX group decreased significantly (P<0.01, P<0.01); Compared with the CTX group, the bone marrow cells and WBC's levels of the Tau +CTX groups(the mudium,high-dose groups) increased (P<0.05, P<0.01).â‘¢Compared with the model group, spleen index and thymus index of the CTX group decreased (P0.05, P<0.05), while compared with the CTX group, spleen index and thymus index of the Tau+CTX groups were improved (P<0.05, P<0.01).â‘£Compared with the model group, NK cell activity of the CTX group decreased (P<0.05); NK cell activity of Tau+CTX groups(the mudium,high-dose groups)were higher than the CTX group(P<0.05, P<0.01).⑤Compared with the model group, T and B lymphocyte proliferation activity of the CTX group had no obvious difference; Compared with the CTX group, T and B lymphocyte proliferation activity of the Tau+CTX groups increased (P<0.05, P<0.01).