Generation Of HSP60-Specific Regulatory T Cell And Effect On Atherosclerosis In Mice

Isolation and functional characterization of CD4+ CD25+ Treg cells from apoE-/- miceObjective To explore the ratio and function of CD4+CD25+T cell in apoE-/- mice and its relation with atherosclerosis. Methods FCA were used to detect and select CD4+CD25+T cell from peripheral blood of apoE-/- mice. The CD4+CD25+T cells'inhibition were investigated through mixed lymphocyte reaction. Cytokines in the supernatant were analysed by ELISA assay. The state of atheromatous plaque were observed. Results Compare to normal mice, CD4+CD25+T cell in apoE-/- mice had similar ratios and activited weaker inhibition and secreted less IL-10 and TGF-β. The plaques of the apoE-/- mice were larger than normal mice. Conclusion CD4+CD25+ T cells in apoE-/- mice lose its suppressive characterization and thus break immunological homeostasis and then cause atherosclerosis. Generation of HSP60-Specific Regulatory T cell and Effect on Atherosclerosis in miceObjective To explore induction of antigen-specific-CD4+CD25+T cell in vitro and its effect on the formation of plaque. Methods Bone marrow monouclear cells were extracted from apoE-/- mouse and cultured into immature dendritic cells by RPM and then were used to induce heat shock protein 60-specific-CD4+CD25+T cells in vitro. FCA were used to detect and select CD4+CD25+T cell. The CD4+CD25+T cells'specific inhibition were investigated through mixed lymphocyte reaction. Cytokines in the supernatant were analysed by ELISA assay. After infusing the CD4+CD25+T cells into homogenous mice, we observed the state of the plaques. Results The expression of co-stimulating factor CD80, CD86 on RPM-treated-dendritic cells was down-regulated. Immature dendritic cells could be used to induce more antigen-specific-CD4+CD25+T cells than mature ones and IL-10 and TGF-βlevels in the culture medium were higher. These CD4+CD25+T cells could suppress the proliferation and the IFN-γproduction of effector T cells in vitro notablly. The plaques of the mice which were infused CD4+CD25+T cells were smaller than those uninfused. Conclusion Immature dendritic cells could be used to induce suppressive antigen-specific-CD4+CD25+T cells which could affect the formation of plaques.