The Reglutation Of Electroacupuncture Therapy On AnnexinA1 In The Rat Cerebral Ischemia Reperfusion

Objective: To explorer the role that Annexin A1 play in the brain ischemia reperfusion, and to observe the mechanism and the possible pathway that electroacupuncture therapy stroke.Methods: S-D male rat was randomized into normal control group, ischemia reperfusion group, electroacupuncture treatment group, DMSO control group, TrkA inhibitor group. MACO model was established by thread embolism. SABC immunohistochemistry was applied to measure the expression change of Annexin A1 in the hippocampus tissue of rat brain. Anatomical points of Shuigou and Chengjiang were selected.K252a, a special tyrosine kinase inhibitor, was used by lateral cerebral ventricle injection to inhibit TrkA.Result (1)In the CA1 region of hippocampus, the expression of Annexin A1 in the ischemia reperfusion group is higher 35.44% than that in the normal control group(P<0.05). The expression of Annexin A1 in the electroacupuncture therapy group is lower 18.96% than in the ischemia reperfusion group(P<0.05); In the CA3 region of hippocampus, the expression of Annexin A1 in the ischemia reperfusion group is higher 44.24% than that in the normal control group(P<0.05). The expression of Annexin A1 in the electroacupuncture therapy group is lower 21.80% than in the ischemia reperfusion group(P<0.05).(2)After TrkA inhibition, in the CA1 region of hippocampus, the expression of Annexin A1 in the K252a group is lower 12.20% than that in the DMSO group(P<0.05). However in the CA3 of hippocampus, the expression of Annexin A1 in the K252a group is higher 9.30% than that in the DMSO group (P<0.05). (3)In the CA3 region of hippocampus, the nuclear expression of Annexin A1 in the ischemia reperfusion group is higher than that in the normal control group(P<0.05) The nuclear expression of Annexin A1 in the electroacupuncture therapy group is lower than in the ischemia reperfusion group(P<0.05).Conclusion: After brain ischemia reperfusion, Annexin A1 expression increase. Electroacupucture can decrease the expression of Annexin A1, electroacupuncture may play a role in the stroke through TrkA to regulate the anti- inflammation function of Annexin A1. And after brain ischemia reperfusion, the nuclear transloction ratio increase, electroacupuncture can reverse the phenomena. Electroacupuncture may alter the fate of neuron apoptosis through the regulation of nuclear transloction of Annexin A1.