| In order to analyze the cytokine IFN-γ, TNF-α, IL-10, IL-6, IL-4, IL-2 in patients with chronic hepatitis B after treatment in serum concentration and its clinical significance, we chose from February 2004 to June 2005 of hepatitis in our hospital clinical research center for conducting clinical trials in patients with HBeAg positive chronic hepatitis B, according to early clinical trials program of treatment with adefovir dipivoxil for 48 weeks. Cytotoxic Bead Array (CBA) in patients with chronic hepatitis B serum cytokine TNF-α, IFN-γ, IL-10, IL-6, IL-4, IL-2 concentration. The results showed that after adefovir dipivoxil treatment, the treatment of 12th weeks ,24th weeks and 48th weeks later, HBV DNA levels, compared with baseline, were significantly decreased (P<0.05), treatment of 12th weeks, 24th weeks, 48th weeks there were no significant difference between each time after treatment. (P>0.05); TNF-α, IFN-γ, IL-10, IL-6, IL-4, IL-2 after treatment for 12th weeks, 24th weeks, 48th weeks of chronic hepatitis B patients the concentration of peripheral blood clearance, compared to the baseline, were significantly elevated (p<0.05), IL-6 in the treatment of 48th weeks decline than 24th week, with statistical significance, while the other five cytokines TNF-α, IFN-γ, IL-10, IL-4, IL-2 treatment for 12Th weeks, 24th weeks and 48th weeks, compared with each other, there are no significant difference (p>0.05); The treatment of 12th weeks when the serum concentration of IL-6 was negatively correlated with HBV DNA, treatment for 24 weeks, In addition to IL-6 outside, IL-10, TNF-αand HBV DNA also a negative correlation (P<0.05), Hepatitis B virus into the body, the body through non-specific immune response and specific immune response followed by resistance to viruses, in virus-specific immune response, whether cell-mediated immunity, or humoral immunity, T lymphocytes are the most important effector, T cells stimulated by virus antigen, the differentiation of T lymphocytes for the destruction (CTL or Tc) and T-lymphocytes (Th), Tc, on the one hand by direct dissolution to remove virus-infected cells, but the main through the secretion of IFN-γ, TNF-αand other cytokines, by way of non-dissolved to remove the virus cells, Th cells secrete Th1 in TNF-α, IFN-γand other cytokines, cell-mediated immunity, Tc cell activation is mediated through Th1 mediated, Th1 dominant response will help clear the virus completely; Th2 cells secrete IL-4, IL-10 and other cytokines, these factors can inhibit Th1-type cytokines in the role of each of these two reactions is the role of anti-hepatitis B virus in the body plays an important role in infection. This article, The treatment of 12th weeks from six cytokines IFN-γ, TNF-α, IL-10, IL-6, IL-4, IL-2 content significantly increased, the time and have a statistically significant increase in baseline (P<0.05), the opposite, HBV DNA levels after 12 th weeks of treatment showed a downward trend, after 24 th weeks, 48 th weeks after treatment, viral load decreased significantly over baseline, with significant difference (P<0.05). Prompted by adefovir dipivoxil treatment, increased levels of cytokines, chronic hepatitis B patients to break the Th1/Th2 cytokine imbalance, the body's immune virus removal capacity is conducive to virus removal. Some cytokines after treatment with anti-virus HBV DNA changes in different time periods have some relevance, in this article, treatment for 12 th weeks at the time of serum IL-6 concentration was negatively correlated with HBV DNA, treatment for 24th weeks, with the exception of IL-6, IL-10, TNF-αand HBV DNA also a negative correlation (P <0.05), The experimental determination of the application of CBA technology at the same time a wide range of patients with chronic hepatitis B serum cytokines, is less common in China, compared with the conventional ELISA, with multiple and sync the advantages of better stability and shorten the duration of the experiment. In short, CBA was able to quickly and accurately the quantitative detection of cytokines; these Th1/Th2 cytokines IFN-γ, TNF-α, IL-10, IL-6, IL-4, IL-2 play an important role in hepatitis B virus infection;⒉taking patients with chronic hepatitis B during adefovir dipivoxil therapy in peripheral blood of IL-2, IL-4, IL-6, IL-10, TNF-α, and IFN-γ, etc. Thl and/Th2-type cytokines in dynamic monitoring of clinical status of workers to understand the immune response, anti-virus and treatment ability to forecast the outcome of a certain practical value. |
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