| Purpose:To prepare aclacinomycin A(ACA)stealth microemulsions(ASM) and evaluate its acute toxicity and anticancer effect in cancer metronomic chemotherapy.Methods:ASM was a kind of transparent solution,in which polylene glycol derivative of distearoylp- sphatidyl ethanolamine(PEG-DSPE) and oleic acid were included.PEG-DSPE was used as a surfactant and to prolong the circulation time of microemulsions in blood while oleic acid was used to form a complex with ACA and increase the loading efficiency of ACA in the microemulsions. ASM was characterized using a laser light scattering instrument,and its loading efficiency was evaluated using a Sephdex G50 column chromatography.Following metronomic chemotherapy, the anticancer effect of ASM was evaluated in animal tumor models.Results:The average diameter of ASM in pure water was 114.5±3.5 nm and its Zeta potential was -30.9±2.0 mv while its loading efficiency was 96。4±0.7%(n=3).The stealth microemulsions prolonged the circulation time of ACA in blood significantly compared with that of ACA while its acute toxicity was much lower than that of ACA.When administrated following metronomic chemotherapy,both ASM and ACA showed greater anticancer effect than that of their corresponding single dose while ASM was more effective than ACA.Conclusions:ASM is less toxic than ACA,and may become a better candidate for cancer metronomic chemotherapy than ACA. |
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