Vitamin D Receptor Gene Polymorphism And Traditional Chinese Medicine Differentiation Type In Relation To Bone Mineral Density In Female Patients With Postmenopausal Osteoporosis

Objective:To study the relationship between the Vitamin D receptor(VDR) gene polymorphism uniting traditional Chinese medicine(TCM) differentiation type and bone mineral density(BMD) in female postmenopausal osteoporosis (POP),and analyze relationship between self-factors and BMD.Methods:①The 180 patients with postmenopausal osteoporoses aged 63±8 years and menopausal age of 45-54 years were investigated through random sampling method in the out-patient clinic and inpatient department,The third affiliated Orthopaedics hospital of Guangzhou university.of traditianal Chinese medicine,during March 2007 to January 2009.There were 116 patients of asdthenic splenonephro-yang,43 of hepatic and renal yin defic-iency and 21 of qi-stagnancy and blood stasis.②The BMD of the 2th-4th lumbar vertebrae,left upper femur,greater troehanter and Ward's area was measured with dual energy X-ray absorptiometry(QDR-4000,Hologic,USA,CV＜0.15%).③Self-factor of patients:Age,menopausal age,menopausal duration,height, body mass and body mineral index were recorded.④The calcitonin receptor(VDR) gene polymorphism was detected by polymerase chain reaction.⑤Normal data were compared with t test,no normal data were compared with Wilcoxon rank sum test,and relationship between self-factors and BMD were analyzed with Bivariate method.Results:Totally 180 POP patients were entered the final analysis.①BMD of POP patients with 3 kinds VDR was not significantly different from that of patients with various TCM differentiation types(p＞0.05),and differences among 3 kinds of TCM differentiation types were not significant(p＞0.05), ②In bb genotype,BMD of the 2th-4th lumbar vertebrae in asdthenic splenonephro-yang patients was significantly lower than that in qistagnancy and blood stasis patients(p＜0.05),but there were no significant differences among other TCM differentiation types(p＞0.05).③Bb genotypos were similar to bb genotype of POP patients in asdthenic splenonephro-yang(p＞0.05).④Among relative factors,age and menopausal duration were negative correlation with BMD at four sites(r=-0.211- -0.439,p＜0.05-0.01);height was positive correlation with BMD in lumbar vertebrae,neck of femur and greater trochanter (r=0.163-0.219,P＜0.05-0.01);body mass was positive correlation with neck of femur(r=0.179,P＜0.05);menopausal age and body mass index were no correlation with BMD(r=0.047-0.161,p＞0.05);age was negative correlation with BMD in lumbar vertebrae and neck of femur(r=-0.347- -0.431,p＜0.05); menopausal age,menopausal duration,height,body mass and body mass index were no correlation with BMD(r=-0.014- -0.247,p＞0.05).Conclusion:①The polymorphism of VDR gene uniting TCM differentiation type is correalted with BMD in POP.②Self-factors such as age,menopausal duration,height and body mass can affect BMD in bb genotype of VDR,and age can also affect BMD in Bb genotype of VDR.