The Effect Of IL-24 Upon Cervical Cancer C33A

Objective:The shuttle vector pAd track CMV that carried the objective gene of IL-24 was transfected into cervical cancer cell line C33A to observe the effect of IL-24 on apoptosis, proliferation and migration in human cervical cancer cell C33A.Method:The eukaryotic expression vector pad treak-cmv/IL-24 and vector padtreak-cmv were transfected into human cervical caneer cell C33A using the lipid body,and the cervical cancer cell C33A as negative control,the pAd track-cmv were transfected into C33A as empty-vector control.The proliferation of C33A was tested by MTT assay.We measured the apoptotic rates by flow cytometry,and the cell migration was detected by cell scratch experiment cell.Result:1.To observe the IL-24 group,the empty-carrier group and the negative control group C33A cells under fluorescence microscope,we observed large amounts cells of the IL-24 group and the empty carrier group emitted green fluorescence,however,the cells of negative control group didn't.2.The OD values showed significant difference among the three groups of cells by MTT assay(P＜0.001,P＜0.001).the OD values of IL-24 group was reduced(P＜0.05),but no significant difference was shown between the empty-vector group and the negative control group(P=0.993). The rowth rate of IL-24- transfected cells was inhibited,while the cell growth curve of empty-carrier group was similar to that of the nagative control group.3.Flow cytometry showed that the apoptotic rates of the negative control group,the empty-carrier transfected group and the IL-24 transfected group were 3.3367%,3.3667%and 13.4700%.The differences of apoptotic rates in C33A among three groups were statistically significant.The IL-24 transfected group resulted in significant tumor apoptosis when compared with that in the negative control group and the empty-transfected group(P＜0.001,P＜0.001), Compared to that of the other two groups,the apoptosis rates of IL-24 group was obviously reduced,but there was no statistically difference between the negative control group and the empty-vector transfected group(P=0.969),By flow cytometry,the cell percentage in G₀/G₁,S and G₂/M period showed significant difference among the three groups(G0/G1:F=77.491, P＜0.001;S:F=8.008,P＜0.05;G2/M:F=10.502,P＜0.05 ).Compared to the other two groups, the number of IL-24 transfected cells in G₀/G₁ period was increased(P_G0/G1＜0.001),cells in S and G₂/M period were reduced(P_S＜0.05;P_G2/M＜0.05),but no significant difference was shown between the empty-vector group and the negative control group(P_S＜0.05).4.compared the widths of scratch of negative control group,empty-carrier transfected group,IL-24transfected group under microscope in cell scratch experimentcell at different times. The cell migration of IL-24 transfected group was restrained,Scratch coalescence of it was off-speed.Conelusions:1.The 1L-24 gene can hinder the C33A cell in G0/G1 scheduled time,and induce apoptosis of human cervical cancer cell C33A2.The IL-24 gene has the effect of guiding the C33A cell to wither to die;3.The IL-24 gene for the cervical cancer gene therapy may have lurking value...