The Effects Of The Hypoxic Training In Different Modes On β-EP In Rats' Hypothalamus And The Leucocyte Counts In Blood

Objective:The purpose of the present study is to investigate the effect of the hypoxic training in different modes on the expression ofβ-ehdorphin gene in ARC and the protein concentration ofβ-EP in rats' hypothalamus,and find out the relationship with the leucocyte counts and the percents of lymphocyte,granulocyte,monocyte.Try to illustratethe influence ofβ-EP on immune function.Method:75 8-week-old male Sprague-Dawley rats,were randomly divided into the following groups:1.living high control group;2.living high and training high group;3.living high and training low group;4.living low control group;5.living low and training low group.The rats were trained with swimming on different altitude in exercise groups basing on the experiment plan.In the morning after the last training,the rats would be Killed,and the expression ofβ-EP gene in ARC,the protein concentration ofβ-EP in rat' s hypothalamus and the sum of leucocyte and the percents of lymphocyte,granulocyte,monocyte in blood were measured.Result:1.Compared with HiCo group,the OD and IOD ofβ-EP gene in ARC were obviously increased in HiHi group and HiLo group in anoxic condition (P<0.05 and P<0.01),and implying the expressing of mRNA in ARC was enhanced.Compared with HiLo group,the OD and IOD of HiHi group were increased(P<0.05 and P<0.01).There was no difference between other groups.2.Compared with LoCo group,the protein concentration ofβ-EP in hypothalamus were distinctly increased(P<0.01) in LoLo group;in anoxic condition,it was enhanced in HiHi group and HiLo group(P<0.05 and P<0.01),and compared with HiLo group,the protein concentration ofβ-EP in hypothalamus was increased(P<0.01) in HiHi group;compared with LoCo group,it was added(P<0.01) in HiCo group,and compared with HiLo group,the protein concentration ofβ-EP in hypothalamus was decreased(P<0.05) in LoLo group.3.Compared with LoCo group,the HiCo group' s sum of leucocyte was obviously decreased(P<0.01),and compared with HiLo group,the LoLo group' s leucocyte counts was evidently increased(P<0.01).There were no statistical changes between other groups. there was no significant changes in the percent of lymphocyte, granulocyte,monocyte in blood.4.in this study,between the protein concentrain ofβ-EP in hypothalamus and the percent of lymphocyte, granulocyte,monocyte had no evidently relationship in different mode training.Conclusion:1.Four weeks experiment later,the changing of the gene expression and the protein concentration ofβ-EP in five group rats behaved HiHi group >HiLo group>HiCo group>LoLo group>LoCo group, but the changing of the protein concentration was more obviously than the changing of gene expression.2.Four weeks experiment later,the leucocyte counts of HiCo group was much lower than LoCo group in rats blood,and that of HiLo group was much lower than LoLo group,there was no significant change in different count of leucocyte.3.Four weeks experiment later,there was no remarkable relationship between the protein concentration ofβ-EP in hypothalamus and the leucocyte count, the modulation mechanism betweenβ-EP and immunity could be knowm by further research in the future.