| Objective: To investigate the levels of serum transforming growth factorβ1 (TGF-β1), tumor necrosis factor (TNF-α) and 24 hours urinary protein (24hUPRO) with IgA nephropathy (IgAN) and Henoch-Schonlein purpura nephritis (HSPN) children . To offer the theoretical evidence and experimental support for new therapia and judgement of the prognosis in the two diseases by the analysis of differences and relationship of the various indicators.Methods: we selected 30 IgAN and 40 HSPN children who came to hebei medical university the second hospital pediatric department from May 2007 to May 2008 as A group and B group. we random selected 20 healthy children as normal control group(C group) in the same Period. HSPN group will be divided into two groups according to 24hUPRO: group 1 of substantial proteinuria and hematuria (24hUPRO≥50mg/Kg) included 19 cases and group 2 of hematuria mainly(24hUPRO<50mg/Kg) included 21 cases. These children who were selected no age differences, no other immune disorders, non-use of glucocor- ticoids and immunodepressant , have normal liver function. The levels of serum TGF-β1 and TNF-αin IgAN , HSPN and normal children was examined by sandwich ELISA methods. The measurement of 24hUPRO is performed by immunonephrelometric. Results were expressed as the mean±SD. The data were analyzed with SPSS13.0 software. Statistical analysis was performed by one-way ANOVA, the linear regression and correlation. P value of less than 0.05 was considered as significant.Results:1 The Compare of the levels of serum TGF-β1 and TNF-αin IgAN, HSPN and normal control group ( X±S):1.1 The level of serum TGF-β1 (pg/ml): There were significant differences among the three groups (F = 29.534,P<0.01). IgAN group : 212.43±73.31 , HSPN group : 167.66±39.85, control group : 100.91±10.28.1.2 The level of serum TNF-α(pg/ml):There were significant differences among the three groups (F=23.249, P<0.01). IgAN group : 19.33±3.48, HSPN group : 16.37±5.74 , control group: 10.51±2.56.2 The Compare of the levels of serum TGF-β1 and TNF-αin group 1 (substantial proteinuria and hematuria) and group 2 (hematuria mainly) ( X±S):2.1 The level of serum TGF-β1 (pg/ml): There were significant differences among the two groups (t = 26.606, P<0.01). group 1: 194.17±32.01, group 2: 141.15±27.50.2.2 The level of serum TNF-α(pg/ml): There were significant differences among the two groups (t = 18.043, P <0.01). group 1: 20.53±5.12, group 2 : 12.21±2.22.3 The correlation of TGF-β1, TNF-αand 24hUPRO in HSPN:3.1 The 24hUPRO was positively correlated with the level of serum TGF-β1 in HSPN(r = 0.714, P<0.01) . The linear regres- sion equation: y=-2.28+0.02x.3.2 The 24hUPRO was positively correlated with the level of serum TNF-αin HSPN( r = 0.743, P<0.01) . The linear regres- sion equation : y = -1.11+0.17x.3.3 The level of serum TGF-β1 was positively correlated with the level of serum TNF-αin HSPN(r = 0.533, P <0.01). The linear regression equation: y = 107.02 +3.70 x.4 The correlation of TGF-β1, TNF-αand 24hUPRO in IgAN4.1 The 24hUPRO was positively correlated with the level of serum TGF-β1 in IgAN( r = 0.508 , P <0.01). The linear regres- sion equation : y=-0.40+0.01x.4.2 The 24hUPRO was positively correlated with the level of serum TNF-αin IgAN (r=0.474 ,P<0.01). The linear regression equation : y=-1.59+0.14x.4.3 The level of serum TGF-β1 level was positively correlated with the level of serum TNF-αin IgAN( r = 0.423 , P<0.01). The linear regression equation : y = 40.30 +8.90x.Conclusion:1. The levels of serum TGF-β1 and TNF-αin IgAN group were significantly higher than those levels in HSPN group. The levels of serum TGF-β1 and TNF-αmay become one of the indicators to early differential diagnosis , initial renal injuries and judgement of the prognosis in the two diseases.2. The levels of serum TGF-β1 and TNF-αin IgAN and HSPN group may effect the progress and prognosis of IgAN and HSPN,so blocking the effects of TGF-β1 and TNF-αwith the antagonists is expected to become the new strategy of glomerular sclerosis and renal fibrosis.3. 24hUPRO can be used as an indicator for dynamic monitoring the renal damage. |
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