Changes Of Osteoglycin, Pâ…  CP In Lung Tissue Plasma In Rabbit Pulmonary Embolism Model

Objective: Study shows that pulmonary embolism resulting in lung injury, reflected primarily in the pulmonary blood flow abnormalities, pulmonary vascular permeability of the microcirculation increase, significantly increased pulmonary vascular resistance, vascular endothelial cell morphology dysfunction, increased permeability, resulting in increased lung water content Oxygenation dysfunction. However, pulmonary embolism on the impact of Interstitial rarely reported. In this study, with HE, transmission electron microscope and Masson staining, we observe morphological changes of lung tissue and interstitial lung; enzyme-linked immunosorbent assay was used to detect the changes of osteoglycin and PⅠCP in lung tissue homogenate, thereby, we can further explore the pulmonary interstitial changes of pulmonary embolism.Method: Forty-eight healthy New Zealand white rabbits of weighing 3.0 ~ 3.5kg were studied in this experiment. The left lower lung artery were obstructed by inflating gas of 5F Berman sacculus catheter to establish rabbit pulmonary embolism model. The animals were randomly divided into six groups: (1) sham- operated group(sham)(n=8),(2) embolism 1h group (n=8), (3) embolism 4h group (n=8), (4) embolism 8h group (n=8), (5) embolism 12h group (n=8), (6) embolism 24h group (n=8), animals in each group were killed on the end point, check the lower left lung tissue to retain a variety of test specimens. HE, transmission electron microscopy and Masson staining were used to observe morphological changes of lung tissue, as well as morphological changes of interstitial lung; enzyme-linked immuneeosorbent assay was used to measure the changes of osteoglycin and PⅠCP in lung tissue homogenate.Results:(1) Pathological changes in lung tissue: HE staining under light microscope showed: lung tissue showed diffuse lesions after pulmonary embolism, and with the embo- lization time extended, the pathological changes gradually become more and more serious. Transmission electron micros- copy revealed that the pathological damage of lung tissue was serious after pulmonary embolism, the number of microvilli on the surface of the type II alveolar cells was decreased, and the osmiophilic multilamellar body was cavitated. Most of cristae and a small number of membrane of mitochondria was to fuse; The expression of collagen in lung was significantly higher in embolization 12h group and embolization 24h group than sham group. That was better in embolization 24h group.(2) Results of Masson staining: in the sham group, there are a small amount of collagen fibers between the epithelial cells and perivascular; embolization 1h, 4h, 8h groups were similar to the sham group, and the contents of collagen fibers and its distribution were also shown little remarkable changes. Compared with the sham group, the content of collagen fibers and its distribution of a remarkable increase in embolization 12h, 24h groups. (3) Changes of osteoglycin in lung tissue homogenate: After acute pulmonary embolism, OGN protein level in lung tissue homogenate was gradually decreased. Compared with the sham group, the OGN protein level of embolization 1h, 4h, 8h groups had no significant difference(10.72±1.06,10.18±0.82,9.79±0.68, P>0.05), embolization 12h, 24h groups decreased significantly (9.09±0.43, 8.21±0.57, P<0.05). (4) Changes of PⅠCP in lung tissue homogenate: After acute pulmonary embolism, PⅠCP protein level in lung tissue homogenate was gradually increased. Compared with the sham group, PⅠCP protein level of embolization 1h, 4h, 8h groups had no significant difference (2.95±0.35,3.18±0.52, 3.42±0.51, P>0.05), embolization 12h, 24h groups increaseed significantly (3.86±0.25,4.28±0.32, P<0.05).Conclusion1 In our experiment, we successfully established pul- monary embolism by using Berman balloon catheter. The experimental animal model of PE owns the advantages in convenient controllability and easy reproducibility.2 HE staining under light microscope showed: lung tissue showed diffuse lesions after pulmonary embolism, and with the embolization time extended, the pathological changes gradually become more and more serious. Transmission electron micro- scope showed that in the processes of pulmonary embolism there exists damage to lung parenchyma, such as type II alveolar epithelial cells and mitochondria, and have obviously interstitial collagen hyperplasia, it suggest that there have interstitial lung changes. The result of masson staining showed that the collagen fibers between the epithelial cells and perivascular were increased in embolization 12h, 24h group.3 The changes of osteoglycin(OGN),PⅠCP content in lung showed that the level of collagen increased at last, and then lead to interstitial lung changes.