The Influence Of Extracellular Matrix Protein On Mitogen-activated Protein Kinase, Phosphatidylinositol 3-Kinase, Protein Kinase C Of Airway Smooth Muscle Cells In Asthmatic Rats

Preface: Airway chronic inflammation and airway reconstitution are importance characters of asthma. As important members of airway wall ,Airway smooth muscle cells and extracellular matrix play important roles in airway chronic inflammation and airway reconstitution of asthma. It is clear that the number and function of airway smooth muscle cell is unlikeliness in asthma to the normal,and the number of extracellular matrix is also distinctness . Extracellular matrix can control the function of Airway smooth muscle cells,but the mechanism is unclear. The primary signal system accelerating the number of ASMCs are Mitogen-activated protein kinase(MAPK), Phosphatidylinositol 3-Kinase(PI3K), Protein kinase C (PKC)signal system.But it is not clear that if extracellular matrix control the function of Airway smooth muscle cells by MAPK,PI3K,PKCsignal system.So we culture the airway smooth cells of asthmatic rats and control rats, seed the ASMCs in flasks which had treaded by extracellular matrix proteinon(conclusion of fironectin,collagen 1,laminin) ,then to delect the gene expression of ERK1/2,PI3K,PKC of the the airway smooth cells by the real time PCR and the protein expression of ERK1/2,PI3Kp85,PKC-aby Western blotting . To observe the influence of extracellular matrix protein on expression of MAPK, PI3K, PKC of airway smooth muscle cells in asthmatic rats.Objective: To observe the influence of extracellular matrix protein on expression of MAPK, PI3K, PKCof airway smooth muscle cells in asthmatic rats.Methods: Twenty-four Wistar rats were divided an asthmatic group (n=12)and a control group (n=12); then culture the airway smooth cells. The airway smooth cells were seeded in cuture flasks which had treaded by extracellular matrix proteinon(conclusion of fironectin,collagen 1,laminin) ,then to delect the gene expression of ERK1/2,PI3Kp85,PKC-aof the the airway smooth cells by the real time PCR and the protein expression of ERK1/2,PI3Kp85,PKC-aby Western blotting .Result: The value of gene expression ofERK1/2,PI3Kp85,PKC-aand protein expression of ERK1/2,PI3Kp85,PKC-ain asthmatic group were higher than the control group (p<0.01) . In asthmatic group, The gene expression relative value of ERK1/2 treated with fironectin , collagen 1,laminin were 7.262±0.614, 3.278±0.037,4.122±0.125;which were higer than asthmatic group untreated with extracellular matrix proteins(1.525±0.054)(p<0.01). The gene expression relative value of PI3Kp85 in asthmatic group treated with fironectin ,collagen 1,laminin were7.626±0.615,9.927±0.122,8.951±0.237;which were higer cpmpared with asthmatic group untreated by extracellular matrix proteins(5.931±0.749)(p<0.01). The gene expression relative value of PKC-a in asthmatic group treated with fironectin ,collagen 1,laminin were7.626±0.615,9.927±0.122,8.951±0.237;which were also higer than those of asthmatic group without treatment(3.478±0.747)(p<0.01). In control group ,The gene expression of ERK1/2,PI3Kp85,PKC-ain ASMCs treated with fironectin ,collagen 1,laminin were indistinctive to that of without treatment(p>0.05).The protein expression of ERK1/2,PI3Kp85,PKC-awere similar to the gene expression in asthmatic group.Conclusions: Extracellular matrix(including of fironectin,collagenⅠ,laminin can make the gene and protein expression of ERK1/2,PKC-α,PI3Kp85 increase in asthmatic rats ASMCs. extracellular matrix protein may control the function of airway smooth muscle cells by MAPK,PI3K and PKC Signal Transduction System.