The Effect Of ShRNA Interference Targeting Hypoxia Inducible Factor-1α On The Expression Of Adhesion Molecule In Human Cervical Carcinoma HeLa Cells

Objective To investigate the effect of shRNA interference(RNAi) targeting hypoxia inducible factor-1α(HIF-1α) on the expression of cell adhesion molecules(CD44v6 and E-cadherin) in human cervical carcinoma HeLa cells.Methods Human cervical carcinoma Hela cell line(marked as H0 cell),transfection pGenesil-1 HeLa cell line(marked as H1 cell)and transfection HIF-1α-shRNA HeLa cell line(marked as H2 cell)were subjected to hypoxia and normal cultivation for different time periods. The expression of HIF-1α,CD44v6,E-cadherin andβ-catenin proteins were measured by Western blotting in each group. The expression of HIF-1α,CD44v6,E-cadherin andβ-catenin in the above 3 groups were detected in transplantation tumors in nude mice by immunohistochemical method.Results Western blotting showed that the expression of HIF-1α,β-catenin and CD44v6 protein was increased and the expression of E-cadherin were decreased in H0 and H1 groups under hypoxic condition. No HIF-1αexpression were found in H0,H1 and H2 groups under normal condition and in H2 group under hypoxic condition. E-cadherin expression was noticed. Immunohistochemical study showed that the expression of HIF-1α,E-cadherin,β-catenin and CD44v6 in the H0 group were(0.651±0.057),(0.198±0.015),(0.465±0.061),and(0.687±0.102),respectively;those in the H1group were (0.671±0.069),(0.201±0.020),(0.452±0.038),and(0.701±0.071),respectively;and those in the H2 group were(0.194±0.023),(0.548±0.045),(0.421±0.052),and(0.187±0.057),respectively.HIF-1α,E-cadherin and CD44v6 expression in H2 group were significantly different from those in the HO andH1 groups(P <0.05). We also noticed that HIF-1αexpression were correlated with E-cadherin and CD44v6 expression.Conclusion shRNA targeting HIF-1αin human cervical carcinoma HeLa cells can down-regulate CD44v6 and up-regulate E-cadherin expression under hypoxia condition.