Testosterone Induce Different-featured Prostate Hyperplasia In Castrated And Uncastrated Rat

ObjectiveBenign Prostatic Hyperplasia is the most common disease in old man in Urology,and interstitial hyperplasia is one of the pathological changes.At present,it is known that androgen has close relation with prostate.Castration or antagonnist can lead to prostate dimification,and extrinsic source androgen can canse to BPH.At present the disequilibrium of prostate cell proliferation and apoptosis is the basis of the BPH.Prostate stroma cell secretion depend on the androgen induction. bFGF cause prostate stroma cell proliferation by paracrine and autocrine modes which lead to prostate hyperblastosis.Bc1-2 is protooncogene that have intimate relation with apoptosis.The mutation and expression inhibition of the bc1-2 can't stimulate cell proliferation,but it can extend the survival time of the not-prolifelation cell.At last it can lead to the increase of absolute number of prostate cells,and the BPH is formed.To study the different features of prostate hyperplasia in castrated and non-castrated rat after testosterone,and we do the animal study.Methods23 SD mail rats were raised adaptability in the laboratory for a week.After a week,all the rats were randomly and equally distributed into 3 groups:normal group (A) as control group,3 rats,whereas castrated(B) and non-castrated rats(C) as study groups,each group 10 rats.The rats in castrated group were anesthetized and were suffered with bilateral orchiectomies in aseptic operation,whereas those in non-castrated groups were only suffered with sham operations.One week later,all rats in two groups were injected through abdominal cavities with testosterone stock solutions as 12.5mg·kg^-1·d^-1 for consecutive 30 days.The control group was given the same volume of normal saline.All rats were sacrificed at the 31 st day.Their ventral and dorsal prostates were observed and weighed individually and were studied by pathological skills on light microscope.After the prostate were weighed,the expression of bFGF and bc1-2 protein was detected by western-blot.ResultsIn experiment,the prosrate hyperplasia were formed by injecting testosterone to castrated and non-castrated rats.The result is that prostate index of two study groups have more increased than the control group,and the distinction have statistical significationIt(p＜0.05),and castrated and non-castrated ones were similar.Larger and higher hyperplasia were discovered in prostatic glands in castrated and non-castrated ones by pathological skills.The expression of bFGF and bc1-2 protein were detected in all groups.It was discoved the expression of bFGF and bc1-2 protein of two study groups were larger than those of the control group(p＜0.05),and castrated and non-castrated ones were similar.It is confirmed that there is high expression of bFGF and bc1-2 protein in prostate hpperplastic tissue.It is explained that prostate cell proliferation and apoptosis have the relation with BPH.ConclusionBoth castrated and non-castrated rats develop prostate hyperplasia after short-term testosterone treatment.Non-castrated rats probably procure an easier and more feasible and reasonable prostate hyperplasia.Pathogenesis in non-castrated group showed more similarities to clinical BPH patients.There is high expression of bFGF and bc1-2 protein in prostate hpperplastic tissue.These discover may help further the studies on the association of castration and androgen with prostate disease.