The Study Of Proteinic Expression On FLT3 And NF-κB Of Acute Myelocytic Leukemia Stem Cell By Using Officinal Herbs

Objective:To research changes of proteinic expression in NF-κB (Nuclear Factor- Kappa B)and FLT3(Fms-like tyrosine kinase 3),and the mechanism of herbs to acute myelocytic leukemia cell(AML).Methods: BM(bone marrow)of forty-five AML patients were extracted to get monocytes .After that, by magneton selecting we can get leukemia stem cells.The third step is cultivating the LSC by four different drugs:Yuanfang group,Fuzheng group,Quxie group and 0.9% NaCl.Then we can obtain protein of LSC. The last is getting the protein expressions by western blotting(WB)and analyzing the changes to summarize the mechanisms of NF-κB and FLT3 by herbs.And observe sex,ages,the WBC and aboriginal cells of BM of AML patients to research the relativity. Results: The expression of FLT3 is not existing differents in different ages and sex of AML patents(P>0.05).But you can get the result that there exists differents in different WBC and aboriginal cells of BM of AML patients(P<0.01). The proportion of FLT3 expression is 62.2 %. The proteinic expressions of NF-κB and FLT3 after different drugs treatment are have dissimilarity between random two groups(P<0.01)except Fuzheng group and Quxie groups(P>0.05). Conclusion: Positive expression of FLT3 in AML patients which are not existing relativity in ages and sex ,but has relativities with WBC and aboriginal cells of BM. Formula Herbs can reduce the exoression of FLT3 and NF-κB. And Yuanfang group can obviously resuce the expression comparing with Fuzheng group and Quxie group. Fuzheng group and Quxie group treatment effect are better when using together.