The Role Of Follicle-stimulating Hormone Receptor-binding Peptide On The Proliferation & Apotosis And Expression Of Survivin In Ovarian Tumer Cells

In recent years,the incidence of ovarian malignancies which have the highest levels of mortality in gynecologic malignancies has been rising.At present,the main therapy of it is still surgical modalities and chemotherapeutics,but the 5-year survival rate for its late stage patient is only about 25%.Therefore,it is an ergent to find a novel and effective therapy and early diagnosis of ovarian malignancies.Epithelial ovarian cancer(EOC) which is the most common malignant ovarian tumors, is often found in postmenopausal or ovarian resection women,and its pathogenesis is not clear yet.Epidemiological studies have shown that pregnancy,childbirth,use of oral contraceptives and breast-feeding can have a significant reduction in ovarian cancer risk.Among the many etiology theory,the continuous ovulation and high gonadotropin theory have been widely recognized.Researches over the past 20 years show that the follicle stimulating hormone(FSH) plays an important role in the carcinogenesis and development of ovarian cancer,but its mechanism and the relationship with the follicle stimulating hormone recepter(FSHR) is still not clear. Some Studies showed that,a conformational receptor recognition site of FSH is related to three binding regions on the FSH molecule.Peptides (Ac-SRAY-NH-(CH₂)₄-CO-TRDL-NH₂) synthesized in two of these binding regions can inhibited FSH-induced cAMP production in Sertoli cells at lower concentrations. Research has also shown that this FSH binding peptides inhibit the growth of ovarian cancer cell significantly.Our experiments are to explore the proliferation,apoptosis and Survivin expression of FSH binding peptides to different ovarian cancer cell line,which may contribute to the etiology and treatment of ovarian cancer research.These experiments were divided into three parts:â‘ the role of FSH binding peptide on the proliferation＆apotosis in ovarian turner cells;â‘¡the role of FSH binding peptide on the expression of survivin in ovarian turner cellsâ‘¢the m olecular m echanism of fsh b inding peptide's role in ovarian turner cell line. Sectionâ… :the role of FSH binding peptides on the proliferation＆apotosis in ovarian turner cellsObjectives To study the role of FSH binding peptides on the proliferation＆apotosis in ovarian tumor cell line SKOV-3,ES-2 and MCV-152Methods Cultured SKOV-3,ES-2 and MCV-152 were treated by FSH binding peptides with different concentrations(0,2.5×10^-12mol/L,2.5×10^-11mol/L,2.5×10^-10mol/L,2.5×10^-9mol/L,2.5×10-8mol/L,2.5×10^-7mol/L,2.5×10-6mol/ L) and 40mIU/ml FSH added 2.5×10^-9mol/L FSH binding peptides in different time. SRB was used to detect the cell proliferation and flow cytometry to examination the cell apoptosis.Results(1) With the concentration of FSH binding peptides increased,MCV-152 and ES-2 cell's proliferation activity compared to those in control have significantly decreased,the differences were statistically significant(P＜0.01).(2) FSH binding fragment can promote ES-2 and SKOV-3 cell apoptosis(P＜0.01). Conclusions FSH binding peptides can inhibit ovarian turner cell line ES-2 and MCV-152 cell proliferation,promote ES-2 cell apoptosis.Sectionâ…¡:the role of FSH binding peptide on the expression of survivin in ovarian turner cellsObjectives to study FSH binding peptide on the expression of survivin in diferenct ovarian turner cells. methods Cultured SKOV-3,ES-2 and MCV-152 were treated by FSH binding peptides with different concentrations(0,2.5×10^-12mol/L,2.5×10^-11mol/L,2.5×10^-10mol/L,2.5×10^-9mol/L,2.5×10-8mol/L,2.5×10^-7mol/L,2.5×10-6mol/ L) and 40mIU/ml FSH added 2.5×10^-9mol/L FSH binding peptides in 24 or 48h, using RT PCR and western blot to detect the survivin mRNA and protein expression.Results With the concentration of FSH binding peptides increased,the expressions of survivin mRNA and protein have significantly decreased.Conclusions follicle-stimulating hormone binding fragment can inhibit ovarian cancer cell line SKOV-3 and ES-2 cells of survivin mRNA and protein expression.ofConclusions FSH binding peptide can inhibit ovarian turner cell with FSHR proliferation and promote apoptosis,decrease the expression of survivinoIn summury,binding fragment of FSHR could inhibit ovarian turner cell proliferation and promote apoptosis,inhibit expression of survivin ovarian of turner cell.The topics for further study the pathogenesis of ovarian cancer has provided new ideas,as well as ovarian cancer new treatments and methods to provide a new target.