The Significance To Detect Twist,P53,E-cadherin Gene In Esophagus Carcinoma

Objective:To detect the expression level of Twist in esophageal cancer,adjacent and normal tissues.At the same time,P53,E-cadherin protein are detected together, further to explore the relationship of Twist and P53,E-cdherin protein with clinic-pathological indicators,and its function and mechanism at the diagnosis and prognosis of malignant tumors.Methods:30 cases of surgical resection specimens in patients of esophageal cancer with pathological diagnosis,which contains 1 case of adenocarcinoma,29 cases of squamous cell carcinoma;Esophageal cancer and adjacent tissues were collected in each of patients,adjacent tissues from the cancer area at the edge of 3-5 cm between the organization,simultaneously to select 10 cases normal esophageal tissues of innocent disease of esophagus in the same period of hospitalization as compared group,specimens were placed in 10%neutral formalin fixed and buried in paraffin, 4μm serial sections standby;All specimens were examined respectively Twist,P53 and E-cadherin protein by SABC immunohistochemical method,and the experimental results were dealed by spss15.0 statistical data analysis package.Results:Twist protein expression in esophageal cancer tissue was significantly higher than adjacent and normal esophageal tissues,adjacent tissues and normal tissues were not significantly different in expression levels;at different depth of tumor invasion,lymph node metastasis,TNM staging and tumor differentiation degree,the expression of Twist protein was significantly different;Expression of P53 and E-cadherin protein in esophageal carcinoma was significantly higher than adjacent tissues and normal esophageal tissues;and at different depth of invasion, lymph node metastasis,TNM staging and varying degrees of tumor differentiation, P53 and E-cadherin protein expression has a significant difference;Twist expression level of esophageal carcinoma has obvious correlation with the existence of P53 and E-cadherin.Conclusion:Twist may be the biological parameter of esophageal diagnosis,as well as determine the course of disease in patients with esophageal disease-specific indicator of prognosis.P53 protein,E-cadherin plays an important role in the process of esophageal cancer growth,invasion and metastasis,Twist probably inhibits apoptosis and promotes tumor cell formation by regulating pathway of ARF/MDM₂/P53,meanwhile impacts expression of E-cadherin to promote situation of EMT,and participates in the occurrence and metastasis of esophageal cancer.