Pharmacodynamics Study Of Yinning Tablets From Thyrine-induced Yin-deficiency Type Of Hyperthyroidism On Animals

BackgroundHyperthyroidism is a clinical common endocrine disease.Chinese medicine theory that the simple goiter is hyperthyroid many areas.Stagnation of liver as a result of the disease and more stagnant heat,so excited Liver,emotion or internal injuries,caused depression of liver.And through its pathogenesis,pregnancy,birth. breast,and other physiological and physical dysfunction,and other factors that have a certain relationship between Yin and Yang in the body is difficult to suppress,poor breathing,mucus plug solidification Adam's apple,if such a long-term,caused by blocked blood agglutination.The beginning of this disease is more,by the disease for a long time to really excess,deficiency,or a mixture.According to the pathological changes of Graves disease is based on pneuma for the first,that is,That is,by the poor breath,sputum clot,blocking blood,hepatic fire,until the yin deficiency,which also involves pathological changes in liver,heart,kidney,spleen,and other organs. That is why we in the treatment of disperse the depressed liver-energy,dissipate phlegm,effects of promoting blood circulation,nourishing Yin and pay attention to the blood and add vitality to adjust the body's organs function to restore the body's normal functions of the various systems,in particular,to restore the body's immune Function,is the only way to effect the consolidation,both temporary and permanent cures.Graves disease in the neck,clinical performance for the enlargement of the neck, sweating span,upset palpitation,polypepsia easy-hungry,impatient initability that vibrate the tongue,physical symptoms such as weight,is a multi-entrails organs, multi-pathogenesis co-exist complex pathological process.Traditional chinese medicine,often through the disperse the depressed liver-energy,purging pathogenic fire,invigotating spleen to remove phlem,calm the nerves,promoting blood circulation and so on a number of ways,many areas of the body in order to achieve the adjustment of the pathological state of imbalance.Chinese medicine and western medicine treatment of hyperthyroidism,and other thyroid diseases have their own advantages,conbined treatment of traditional chinese medicine and western medicine-based comprehensive treatment,clinical research in recent years has become one of the important issues,from a large number of chinese and western medicine treatment can be seen,traditional Chinese and western medicine treatment of thyroid disease is a general trend,over the past 10 years in Chinese medicine treatment of hyperthyroidism has been encouraging progress, dialectical classification,treatment means of flexible and diverse,certainly effect, especially in improving symptoms,regulating the body's immune mechanism to reduce the side effects of western medicine the advantages of larger,more developed a summary of the new prescription treatment.Dialectical Chinese medicine diagnosis and treatment of thyroid function to promote the recovery,not only accepted by the majority of patients,and many cases have been confirmed to improve the clinical efficacy,but also the convenience of patients.Graves of clinical research and scientific experiments in the depth and breadth of progress on both the future needs of immunology,molecular biology,biochemistry, gene engineering and so on new technologies and new methods to strengthen the hyperthyroidism drug ingredients,structural transformation of the study,selected from those used to better effect in clinical treatment in order to obtain better results.Yinning tablets as a result of on-chip Guangzhou Hospital of the South side of clinical experience,type of YIN-deficiency,phlegm-damp and blood stasis hyperthyroidism have a better clinical effect.This study will be the subject combination of pharmacy and pharmacology,endocrinology,nuclear medicine, histopathology,health statistics,such as study methods and techniques,as a result of the Yinning tablet efficacy of clinical research to achieve security for the purpose of the treatment of hyperthyroidism,while the need to adapt to the approval of new drugs. Through the system to specific experimental studies of traditional Chinese medicine as a result of Yinning tablet to evaluate the efficacy. The main contentPurpose1 To establish the model of Yin-deficiency hyperthyroidism by fed rat with thyrine. and compare the different rat model of gender differences.2 Observe the effect of Yinning tablets on the treatment of in hyperthyroidism Yin-deficiency rat or mice Pharmacodynamic role of the Yin deficiency hyperthyroidism-value of serum triiodothyronine(T₃),thyroxine(T₄),free of the original three-iodine thyroid Acid(FT₃),free thyroxine(FT₄),thyroid stimulating hormone(TSH) and the appearance of rats or mice body weight,rectal temperature and pathology testing and analysis.3 Yinning as a result of the tablet for Toxicological Research,observed in 1 day with the largest dose oral suspension given to mice,generated by the acute toxicity and death,for the clinical application of the provision of reliable theoretical basis and protection.Methods1 Model of Yin-deficiency in rat hyperthyroidismSD rats get separated from each group were randomly divided into 4 by gender,8 groups of 6,Group 1 for the control group,2 to 4 for the experimental group of drug-treated(that is,the film thyroid saline suspension Liquid low-,medium-and high-dose group).In addition to Group 1 intragastric administration given volume of normal saline,the other group were given the concentration of 2.5 mg.kg^-1,5 mg.kg^-1, 10mg.kg^-1 the suspension Thyroid tablets saline,once a day,administration 30d in a row after rats were killed.Observation model rats in each group after the appearance, body weight,rectal temperature changes,and the value of serum T₃,T₄,FT₃,FT₄, TSH test,compared with the same time,differences in male and female rats.2 Yinning tablets of hyperthyroidism as a result of Yin-deficiency of thyroid function in rats or mice,as well as the appearance of pathological effectsTake SD female rats or mice 48,according to the administration on an empty stomach weight,were randomly divided into 6 groups of 8,namely:saline group, model group hyperthyroidism,graves ling group,as a result of on-chip low-yinning, Medium and high-dose group.In addition to the normal saline group,the other groups intragastric administration of once a day 10mg.kg^-1/20mg.kg^-1 suspension of thyroid tablets,30d in a row for hyperthyroidism-deficiency model of preparation,modeling the first 31 days intragastric administration once a day,graves ling group 0.6g.kg^-1 /1.2g.kg^-1 tablets suspension,as a result of on-chip low-yinning,middle and high-dose group were given 0.3g.kg^-1,0.6g.kg^-1,1.2g.kg^-1/0.6g.kg^-1,1.2g.kg^-1,1.8g.kg^-1as a result of the suspension tablet yinning,model control group and control group given saline capacity,and so on,for the treatment of rats or mice were killed after 30d,the RIA-free and put rats or mice in each group determined the value of serum T₃,T₄, FT₃,FT₄,TSH,and to observe the body weight,rectal temperature changes in behavior and appearance,taking thyroid gland,adrenal gland,liver weighing;And through organizations to observe the pathological changes in white blood testing,the total number of cells in the tablets.3 Acute toxicityTo mice ted with yinning tablet as a result of on-chip preparation of the largest concentration of the suspension.did not detect LD₅₀,pretending to be the largest amount of drug testing.Take the male and female Kunming mice 20,according to the administration on an empty stomach weight,depending on body weight divided equally between the 2 groups by gender:normal control group and treatment group(yinning tablet group), as a result of the treatment group as to the preparation of yinning tablet into the largest concentration of the suspension 0.3g.ml^-1,12g.kg^-1 dose of intragastric administration,2nd day,2nd interval delivery 4h,the control group given the same volume of saline.7 days after the delivery of animals to observe the performance of the various symptoms of poisoning and death records of the number of animals.Results1 Thyroid tablets to give oral suspension 30d,the experimental treatment group compared with the saline group,with the exception of group 4 in female rats serum T₃, T₄,FT₃,FT₄ value significantly increased(P＜0.01),serum TSH was significantly lower(P＜0.05),the other group were not significantly different(P＞0.05),that Group 4 successful model in female rats and in female than male rats more easily prepared by hyperthyroidism-deficiency rat model.2 Yin-deficiency in rat or mice hyperthyroidism on the treatment of drug-30d, hyperthyroidism and model group,hyperthyroidism ling group,as a result of on-chip low-yinning,middle and high-dose group of serum T₃,T₄,FT₃,FT₄ value and rectal temperature were significantly lower(P＜0.05),serum TSH value,weight,white blood cells and neutrophils significantly increased the total number(P＜0.05),as a result of that tablet have a yinning-deficiency treatment of hyperthyroidism in rats or mice.3 Acute toxicity tests are the result of mice ted with yinning as a result of tablet one day,the largest amount of tolerance for 24g.kg^-1,the equivalent of 60kg adult on the amount of 400 times,7d mice all survived,took place without any adverse reactions.Conclusion1 Through an indicator of the rats to determine the intragastric administration with thyroid Suspension 10mg.kg^-1,for 30d after female rats hyperthyroid rat-deficiency model successful.2 As a effect of hyperthyroidism can yinning Yin-deficiency rats or mice serum T₃, T₄,FT₃,FT₄,TSH of the normal value,but also to increase its weight,lower rectal temperature,to improve the appearance,and the effectiveness of its ling similar to hyperthyroidism.3 As a effect of Yinning's maximum tolerated dose experiment to prove mice without acute toxicity.