Research On The Effects Of Bushen Yifei Jiedu Recipe And Expressing Of P53 And Survivin In Lung Carcinoma Rat Model

Objective:To observe and investigate the effect and mechanism of Chinese herbal compound Bushenyifeijiedu recipe on rat with induced lung cacinoma as well as lung-kidney vacuityrat with induced lung caner,and to obsevre the effects of Bushenyifeijiedu recipe expression of p53 and survivin in model mice.in order to provide scientific evidence for the clinical application of Bushenyifeijiedu(BSYFJD)recipe on the prevention of lung carcinoma especially lung-kidney vacuity type.Methods:Wista Rats were divided randomly by 6 groups:disease model group,high dose of BSYFJD for disease group,low dose of BSYFJD for disease group,diease patten model group,high dose of BSYFJD for disease patten group,low dose of BSYFJD for disease patten group..Rats' model of Induced lung carcinoma was set up by intra-left lobar-bronchial instillation with carcerogen.Mouse model of lung-kidney vacuity type was set up by hanging, smoking,swimming.The effect of BSYFJD recipe on normal condition of rat with induced lung carcinoma as well as lung-kidney vacuity rat with induced lung cancer was observed.Weight of lung and spleen was measured.Every stage of canceration of lung was observed by making pathological section.Expressing of p53 and survivin by immunohistochemical method.Results:After inducing some rats appear lung carcinoma while some appear squamous metaplasia of bronchus.The incidence of lung carcinoma(including precancerous lesion) in model group is higher than drug group.BSYFJD recipe can significantly inhibit the incidence of inducing lung carcinoma and inducing lung-kidney vacuity type lung carcinoma on rats, compared with disease group and disease patten group(P＜0.05).It could release the pathological condition of lung,protect the immune organ and improve the rats' immunity function, particularly for the high-dose group(P＜0.05).Compared to disease model group,high-dose BSYFJD recipe could decrease the expression of p53,survivin(P＜0.05) of cancerous tissue of rats with inducing lung cancer.Compared to disease patten model group,neither high nor low dose BSYFJD recipe could decrease the expression of p53(P＞0.05) of cancerous tissue of lung-kidney vacuity rat with induced lung caner,but it could inhibit the loss of the expression of survivin(P＜0.05).Conclusion:Bushenyifeijiedu recipe could significantly inhibit the incidence of lung carcinoma on rats.The effect of BSYFJD recipe on lung-kidney vacuity rat with induced lung cancer was also significant.The related mechanism may be that BSYFJD recipe can improve the immunity function of rats,release the pathological degree of lung and decrease the expression of p53 and survivin.