Study On The Active Ingredient From S. Tamariscina (Beauv.) Spring.

This dissertation is composed of four chapters.The first chapter reported the extracting procedure of amentoflavone from S.tamariscina(Beauv.) Spring.The second chapter showed the inhibitory activity of acidic extract,ethyl acetate extract and amentoflavone toα-glucosidase.The third chapter included two parts, the first part told the effect of the acidic extract,ethyl acetate extract and amentoflavone on serum TC and TG in hyperlipidemia mice,another part introduced the effect of amentoflavone on serum GOP and GPT in the liver-injured mice.The fourth chapter summarized the studies on Selaginella tamariscina and amentoflavone presently.Chapter 1.Extracting procedure of arnentoflavone from Selaginella tamariscinaAlkali extraction and acid deposition was chosen as the optimum extracting method and the mass fraction of amentoflavone was detected by HPLC as the index of examination evaluated extraction process from Selaginella tamariscina. The optimum technological conditions,were determined using the single factor and orthogonal experiment,were 2 mg/mL ofρ(NaOH),1:40[m(extract solutions): m(Selaginella tamariscina)],50℃(extraction temperature) and 30 min(extraction time).The optimum value of pH was 3.The mass fraction of amentoflavone in the extract was 10%～12%after extraction one time. Chapter 2.The inhibitory activity of different parts of Selaginella tamariscina toα-glucosidaseThis chapter studied the inhibitory activity of the acidic extract,ethyl acetate extract and amentoflavone toα-glucosidase.The results showed the inhibitory activity of the three parts were better than that of acrbose,and their value of IC₅₀ (IC₅₀ of acidic extract=159.49μg·mL^-1,IC₅₀ of ethyl acetate extract=4.8557μg·mL^-1,IC₅₀ of amentoflavone=7.6537μg·mL^-1) were lower than that of acrbose (IC50=1081.27μg/mL).Chapter 3.The endosomatic activity of different parts of Selaginella tamariscinaThe third chapter included two parts,the first part told that the acidic extract,ethyl acetate extract and amentoflavone could distinctly depress serum TC and TG in hyperlipidemia mice,another part introduced that amentoflavone could distinctly depress serum GOP and GPT in the liver-injured mice.Chapter 4.Overview of Selaginella tamariscinaThis chapter is composed of two parts.The first part showed the progress of Selaginella tamariscina on both chemical composition and pharnacological activities in the decades:there were lots of flavonoids and phenolic compounds in Selaginella tamariscina,which showed good activities in anti-tumor and immunity etal.The second part summaried the studies on plant sources,pharmacological activity and extracting procedure of amentoflavone.