The Experimental Study Of The Expression Of Estrogen Receptor α And β In Malignant Tumor Of Salivary Gland

【Objective】To investigate the expression of estrogen receptorα(ERα) and estrogen receptorβ(ERβ) in malignant tumor of salivary gland,to detect the distribution of ERa and ERβbetween these clinical indexes,such as the sex of patients,the age of patients,the size of tumor and lymph move of node,and to detect whether there is a dependablity between the expression of ERαand ERβin amount.The significance lies in discussing the possibility and mechanism of the hormone therapy to the malignant tumor of salivary gland.【Methods】A total of 100 paraffin sections were taken out from the post-operation paraffin sections of salivary gland disease in 2003-2009,including 20 malignant mixed tumors,22 adenoid cystic carcinomas,28 mucoepidermoid tumors,10 duct carcinomas,and matching 20 samples of inflammation of salivary gland. Immunohischemistry was used to measure the expression of ERαand ERβin different cases.The standard of positive result immunoreactivity was scored as negative if 10% or less of tumor cells showed scattered faint staining and positive if more than 10%of cells had strong staining.About statistics,we used SPSS13.0 to analysis data by x~2 test.【Results】(1)The positive rate of ERαis 20%in matching cases and 25%in malignant tumor cases with no difference.The positive rate of ERβis 90%in malignant tumor cases and it is higher than that of matching cases.(2)In different pathologic types of malignant tumor,the expression of ERαhas no difference in statistics,so does the expression of ERβ.(3)In different positions of malignant tumor, the expression of ERαhas no difference in statistics,so does the expression of ERβ.(4) About clinical indexes,in statistics,the expression of ER has a difference in sex groups,has no difference in age groups,and has no difference in the groups,which were classified by lymphatic metastasis condition.(5)in malignant tumor of salivary gland,the positive rate of ERβis higher than ERα,and their expression have no dependablity in amount.【Conclusions】1.There is a general expression of ER in salivary malignant tumor. ERαlocated in cell nucleus and ERβlocated in cytolymph predominantly.2.In different pathologic types and positions of malignant tumor,the expression of ERαhas no difference,so does the expression of ERβ.3.In different age groups,the expression of ER is identical on the whole,and the expression of ER is identical no matter the malignant tumors have lymphatic metastasis or not,but the expression of ER has a difference in sexes.4.Compared to ERα,ERβmay play a more important role in the occurrence and development of malignant tumor of salivary gland.When the estrogen regulates the biologic behavior of salivary gland malignant tumor,the two estrogen receptor isoforms may impact the regulating process by a series of cross-linked factor.