Anti-tumor Effects Of Isomalabaricane Triterpenes On Transplant Subcutaneous Sarcoma In S₁₈₀ Mice

Objective:To explore the anti-tumor effect of Isomalabaricane triterpenes on trsnsplant subcutaneous sarcoma in S₁₈₀ mice.Methods:The subcutaneous transplnantable tumor model of S₁₈₀ sarcoma in KM mice were established and divided at random into five groups:(0.1,0.5,1.0mg/ml) Isomalabaricane triterpenes, blank group(edible oil)and CTX(25mg / kg)group,each of which was composed of nine KM mice and received medicine(oil) in the following five days,interval for two days and continue five days again.The mouse was killed by dislocation of cervical spine surgery 24 hours after medication.The inhibition rate(TIR) was calculated to evaluate the anti-tumor effect by checking tumors'weight.The apoptotic index(AI) was determinated by the terminal deoxynucleotidyl transferase-mediated nick end labeling method(TUNEL).The Caspase-3 with the Bcl-2 gene protein expression in tumor tissue was determinated by the Immunohistochemical method.Results:Isomalabaricane triterpenes can inhibit tumor occurrence and development effectively,with a dose-dependent manner,the high-dose group's and Cyclophosphamide group' s inhibition rate are 40.6%and 58.2%,respecting compared with blank control group,there were statistically significant (P＜0.01).However,high-dose group and the cyclophosphamide group compared with each other the inhibition rate were no significant difference(P＞0.01);Low-dose group's and intermediate dose group's inhibition rates were 29.1%and 24.7%,There is no significant difference between the two groups.Isomalabaricane triterpenes could induce tumor cell apoptosis,high,middle and low dose groups'apoptotic index were 11.04%,9.12%and 8.85%.Comparison of the three groups can be seen among high-dose group's apoptotic index are more statistically significant than the middle dose group's and low-dose group's.Isomalabaricane triterpenes could increased Caspase-3 gene activity. At the same time,inhibiting the activity of Bcl-2 gene significantly,especially in the high dose groupConclusion:Isomalabaricane triterpenes can inhibit the S₁₈₀ sarcoma cell s growth.By increasing the activity of Caspase-3 and reduction of Bcl-2 activity induced apoptosis in tumor cells.